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...7.5; 2 mM EDTA),magnetically collected and resuspended in 25 µl 2X BINDING BUFFER. ...re magnetically collected, the 2x BINDING BUFFER is removed and 50 µl WASH BUFFER (0.15 M LiCl; 10 mM Tris pH 7.5; 1 mM EDTA) is added. The wash procedure is ...