Genome Assemblies: Difference between revisions

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=== '''Assembly_2.1(Spur_2.1)'''===
=== '''Assembly_2.1(Spur_2.1)'''===
=== '''Assembly_0.5(Spur_0.5)'''===
=== '''Assembly_0.5(Spur_0.5)'''===
== '''''Patiria miniata''''' ==
=== '''V2.0 Assembly'''===
We sought to improve the Patiria miniata genome assembly with additional PacBio sequences. We generated a new PacBio read dataset at the Duke University Sequencing Center using our reference individual DNA. The read dataset contains 2 million reads and 15.8 billion bp. The read N50 is 10.4 Kb. We used PBJelly2 to combine the PacBio reads with the previously assembled contigs. The results were an improvement in contig size and number with only a small reduction in the number of scaffolds (Table). The P. miniata Gene v2.0 set was generated using MAKER2 pipeline from v2.0 genome assembly.
Pm v1.0 Pm v2.0
Scaffold number 60,183 57,698
Scaffold N50 52,6141 76,341
Contig number 179,756 131,779
Contig N50 9,466 18,676
<table style="width:100%">
  <tr>
    <th>Firstname</th>
    <th>Lastname</th>
    <th>Age</th>
  </tr>
  <tr>
    <td>Jill</td>
    <td>Smith</td>
    <td>50</td>
  </tr>
  <tr>
    <td>Eve</td>
    <td>Jackson</td>
    <td>94</td>
  </tr>
  <tr>
    <td>John</td>
    <td>Doe</td>
    <td>80</td>
  </tr>
</table>
=== '''V1.0 Assembly'''===
== '''''Lytechinus variegatus''''' ==
=== '''Assembly LvPtE5C'''===
=== '''Assembly LvMSCB'''===
=== '''Assembly 2.2 (Lvar_2.2)'''===
=== '''Assembly 0.4 (Lvar_0.4)'''===

Revision as of 14:21, 4 December 2019

Echinoderm Genome Assemblies by Species


Strongylocentrotus purpuratus

Assembly_3.1 (Spur_3.1)

Assembly 2.6(Spur 2.6)

Assembly_2.5(Spur_2.5)

Assembly_2.1(Spur_2.1)

Assembly_0.5(Spur_0.5)

Patiria miniata

V2.0 Assembly

We sought to improve the Patiria miniata genome assembly with additional PacBio sequences. We generated a new PacBio read dataset at the Duke University Sequencing Center using our reference individual DNA. The read dataset contains 2 million reads and 15.8 billion bp. The read N50 is 10.4 Kb. We used PBJelly2 to combine the PacBio reads with the previously assembled contigs. The results were an improvement in contig size and number with only a small reduction in the number of scaffolds (Table). The P. miniata Gene v2.0 set was generated using MAKER2 pipeline from v2.0 genome assembly.


Pm v1.0 Pm v2.0 Scaffold number 60,183 57,698 Scaffold N50 52,6141 76,341 Contig number 179,756 131,779 Contig N50 9,466 18,676

Firstname Lastname Age
Jill Smith 50
Eve Jackson 94
John Doe 80

V1.0 Assembly

Lytechinus variegatus

Assembly LvPtE5C

Assembly LvMSCB

Assembly 2.2 (Lvar_2.2)

Assembly 0.4 (Lvar_0.4)