EchinoWiki - User contributions [en]

File:Filter coordinates.jpg

2023-10-06T15:29:05Z

Xenbase: Xenbase uploaded File:Filter coordinates.jpg

Image to show how filter coordinates are determined.

Epgfp

2023-10-06T15:11:39Z

Xenbase:

<p>The plasmid vector used as a vehicle to test the cis-regulatory function of randomly cloned genomic DNA fragments was modified from a previous construct, EpGFP (Arnone et al., 1997; Arnone et al., 1998). The grand-parent of this plasmid was pCMV (Clontech); it has ampicillin resistance. EpGFP contains the region around the start of transcription of the endo16 gene (from -117 to +20).The activity of this element has been described in detail elsewhere (Yuh and Davidson, 1996; Yuh et al., 1996,1998, 2001). In addition to a TATA-containing basal promoter, it contains auxiliary sites for a ubiquitous sea urchin transcription factor which promotes DNA looping and has weak transcriptional enhancement activity(GCF1; Zeller et al., 1995). The vector also includes a sea urchin ribosome binding site from the cyIIa gene (Arnone et al., 1998), positioned at the start of the coding sequence of the [http://www.fpbase.org/protein/gfp-s65t/ GFP (S65T)] reporter. The original EpGFP construct was further modified by the addition of a double-stranded oligonucleotide polylinker inserted between the MluI and XmaI sites in the Multiple Cloning Site (MCS), so that the MCS contains the following restriction enzyme sites: 5'-KpnI-SacI-MluI-EcoRI-SpeI-BglII-SmaI/XmaI-3', situated just upstream of the endo16 basal promoter.</p>

== EpGFPII ==



<div class="toccolours mw-collapsible mw-collapsed" style="clear:both">
EpGFPII FASTA Sequence
<div class="mw-collapsible-content">>epgfpii
<pre>
1 11 21 31 41 51 61 71 81 90
| | | | | | | | | |
GGTACCGAGC TCTTACGCGT GAATTCACTA GTAGATCTCC CGGGTTAAAC TGTTTGAGTT TCGTCTCCTG ATTGTGCTAT CAAAGACAAA 90 epgfpii
GGGGTGTAAC TTTACCCCCC TCATCAAGAG CGGAGGGTTA AATAGAGAAA GACTGGTCGA GGACAGGTCA TAATATTGCT AATTTTTAAG 180 epgfpii
CTTATCATCA TGTGTGACGA CGACGTCGCC GCTCTTGTCG TCGACAACGG ATCTGCGGCC GCCGCCACCA TGAGCAAGGG CGAGGAACTG 270 epgfpii
TTCACTGGCG TGGTCCCAAT TCTCGTGGAA CTGGATGGCG ATGTGAATGG GCACAAATTT TCTGTCAGCG GAGAGGGTGA AGGTGATGCC 360 epgfpii
ACATACGGAA AGCTCACCCT GAAATTCATC TGCACCACTG GAAAGCTCCC TGTGCCATGG CCAACACTGG TCACTACCTT CACCTATGGC 450 epgfpii
GTGCAGTGCT TTTCCAGATA CCCAGACCAT ATGAAGCAGC ATGACTTTTT CAAGAGCGCC ATGCCCGAGG GCTATGTGCA GGAGAGAACC 540 epgfpii
ATCTTTTTCA AAGATGACGG GAACTACAAG ACCCGCGCTG AAGTCAAGTT CGAAGGTGAC ACCCTGGTGA ATAGAATCGA GCTGAAGGGC 630 epgfpii
ATTGACTTTA AGGAGGATGG AAACATTCTC GGCCACAAGC TGGAATACAA CTATAACTCC CACAATGTGT ACATCATGGC CGACAAGCAA 720 epgfpii
AAGAATGGCA TCAAGGTCAA CTTCAAGATC AGACACAACA TTGAGGATGG ATCCGTGCAG CTGGCCGACC ATTATCAACA GAACACTCCA 810 epgfpii
ATCGGCGACG GCCCTGTGCT CCTCCCAGAC AACCATTACC TGTCCACCCA GTCTGCCCTG TCTAAAGATC CCAACGAAAA GAGAGACCAC 900 epgfpii
ATGGTCCTGC TGGAGTTTGT GACCGCTGCT GGGATCACAC ATGGCATGGA CGAGCTGTAC AAGTGAGCGG CCGCGGCTCG AGGCTCTAGA 990 epgfpii
GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 1080 epgfpii
TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA 1170 epgfpii
TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG 1260 epgfpii
ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC 1350 epgfpii
ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 1440 epgfpii
ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 1530 epgfpii
CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1620 epgfpii
GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1710 epgfpii
CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1800 epgfpii
CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1890 epgfpii
ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1980 epgfpii
GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 2070 epgfpii
ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 2160 epgfpii
TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 2250 epgfpii
CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 2340 epgfpii
TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 2430 epgfpii
ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2520 epgfpii
CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 2610 epgfpii
TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2700 epgfpii
ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 2790 epgfpii
AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2880 epgfpii
AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 2970 epgfpii
TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 3060 epgfpii
TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 3150 epgfpii
AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 3240 epgfpii
ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG 3330 epgfpii
CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 3420 epgfpii
TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 3510 epgfpii
CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 3600 epgfpii
GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 3690 epgfpii
TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT CCCATTCGCC 3780 epgfpii
ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCCCAAGCTA CCATGATAAG TAAGTAATAT 3870 epgfpii
TAAGGTACGG GAGGTACTTG GAGCGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA 3960 epgfpii
CTAACATACG CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT 4050 epgfpii
CTCTATCGAT A 4061 epgfpii
</pre></div>
</div>
[http://ftp.echinobase.org/pub/WikiDownloads/epgfpii.fasta Download FASTA file for EpGFPII]

=== REFERENCES ===

Yuh, C. H., Moore, J. G. and Davidson, E. H. Quantitative functional interrelations within the cis-regulatory system of the S. purpuratus Endo16 gene. Development 122, 4045-4056, 1996.<span class="newwin">[https://www.echinobase.org/literature/article.do?method=display&articleId=36543 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H. and Davidson, E. H. Modular cis-regulatory organization of Endo16, a gut-specific gene of the sea urchin embryo. Development 122, 1069-1082, 1996.<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36421 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H., Bolouri, H. and Davidson, E. H. Genomic cis-regulatory logic: Functional analysis and computational model of a sea urchin gene control system. Science 279, 1896-1902, 1998.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36860 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H., Bolouri, H. and Davidson, E. H. cis-Regulatory logic in the endo16 gene: Switching from a specification to a differentiation mode of control. Development 128, 617-628, 2001.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=37679 [open<nowiki>]</nowiki>]</span>

Zeller, R. W., Coffman, J. A., Harrington, M. G., Britten, R. J. and Davidson, E. H. SpGCF1, a sea urchin embryo transcription factor, exists as five nested variants encoded by a single mRNA. Dev. Biol. 169, 713-727, 1995.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=35795 [open<nowiki>]</nowiki>]</span>

Arnone, M. and Davidson, E. H. The hardwiring of development: Organization and function of genomic regulatory systems. Development 124, 1851-1864, 1997.

Arnone, M. I., Martin, E. L. and Davidson. E. H. Cis-regulation downstream of cell type specification: A single compact element controls the complex expression of the CyIIa gene in sea urchin embryos. Development 125, 1381-1395, 1998.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36858 [open<nowiki>]</nowiki>]</span>

File:DIGscreen image3.png

2023-10-06T15:06:55Z

Xenbase:

File:DIGscreen image2.png

2023-10-06T15:06:35Z

Xenbase:

File:Digscreen image1.png

2023-10-06T15:06:15Z

Xenbase:

DIGscreen

2023-10-06T15:05:54Z

Xenbase:

'''<span style="font-size:18.0pt;line-height:107%">Protocol for Genomic Library Screening </span>'''

=1. PCR DIG Labeling =

The DIG Nonradioactive System from Roche is the basis for our screening protocol. It is a sensitive method for nucleic acid labeling and detection. The labeled probe can be used to detect target nucleic acid (RNA or DNA) on a membrane (Southern, Northern, or dot blot).

''' '''

This is the standard method, for alternative labeling method, please see #2.

(For reagent and more details, please refer to the [https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/287/736/dig-application-manual-for-nonradioactive-in-situ-hybridisation-iris.pdf kit protocol].)

For each experimental or control sample, add the following components to a sterile microcentrifuge tube. Place the tube on ice during pipetting.

Steps:

<span style="line-height:111%">1)<span style="font:7.0pt "Times New Roman""> </span></span>Thaw the reagents and store on ice;

Briefly vortex and centrifuge all reagents before setting up the reactions.

<span style="line-height:149%">2)<span style="font:7.0pt "Times New Roman""> </span></span>Prepare a 10X concentration solution (1-10ul) of the forward and reverse PCR primer

<span style="line-height:111%">3)<span style="font:7.0pt "Times New Roman""> </span></span>For each 50ul reaction, add the components in the order listed below to a sterile reaction tube on ice

{|
!width="20%"| '''<span style="font-size:11.0pt;line-height:107%"><span style="mso-spacerun:yes">Reagent </span></span>'''
!width="20%"| '''<span style="font-size:11.0pt;line-height:107%">Volume Required for DIG-labeled experimental probe </span>'''
!width="20%"| '''<span style="font-size:11.0pt;line-height:107%">Volumne Required for Unlabeled control probe </span>'''
!width="20%"| '''<span style="font-size:11.0pt;line-height:
107%">Volume Required for DIG labeled <span class="SpellE">tPA</span> control probe </span>'''
!width="20%"| '''<span style="font-size:11.0pt;line-height:107%"><span style="mso-spacerun:yes">Final concentration </span></span>'''
|-
| <span style="font-size:11.0pt;line-height:
107%">Water PCR-grade </span>
| <span style="font-size:
11.0pt;line-height:107%">Add up to 50ul </span>
| <span style="font-size:11.0pt;line-height:
107%">Add up to 50ul </span>
| <span style="font-size:11.0pt;line-height:
107%">Add up to 50ul </span>
| <span style="font-size:11.0pt;line-height:
107%"><span style="mso-spacerun:yes"> </span></span>
|-
|
<span style="font-size:11.0pt;line-height:
107%">PCR buffer w/ </span>

<span style="font-size:11.0pt;line-height:
107%">MgCl2 (10X) </span>

<span style="font-size:11.0pt;line-height:
107%">(vial3) </span>
| <span style="font-size:
11.0pt;line-height:107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">1X </span>
|-
| <span style="font-size:11.0pt;line-height:
107%">PCR DIG probe synthesis Mix (vial2) </span>
| <span style="font-size:
11.0pt;line-height:107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">- </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
|
<span style="font-size:11.0pt;line-height:99%">200uM <span class="SpellE">dATP,dCTP,dGTP</span>, 130uM dTTP,70 <span class="SpellE">uM</span> </span>

<span style="font-size:11.0pt;line-height:
107%">DIG-<span class="SpellE">dUTP</span> </span>
|-
|
<span class="SpellE"><span style="font-size:
11.0pt;line-height:107%">dNTP</span></span><span style="font-size:11.0pt;
line-height:107%"> stock </span>

<span style="font-size:11.0pt;line-height:
107%">solution (vial4) </span>
| <span style="font-size:
11.0pt;line-height:107%">- </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">- </span>
| <span style="font-size:11.0pt;line-height:
107%">200uM each <span class="SpellE">dNTP</span> </span>
|-
| <span style="font-size:11.0pt;line-height:
107%">Forward PCR </span>
| <span style="font-size:
11.0pt;line-height:107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">0.1-1uM </span>
|-
| <span style="font-size:11.0pt;line-height:
107%">primer 10X conc. </span>
|

|

|

|

|-
| <span style="font-size:11.0pt;line-height:
107%">Reverse PCR primer 10X </span>
| <span style="font-size:
11.0pt;line-height:107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
| <span style="font-size:11.0pt;line-height:
107%"><span style="mso-spacerun:yes"> </span></span>
| <span style="font-size:11.0pt;line-height:
107%">0.1-1uM </span>
|-
|
<span style="font-size:11.0pt;line-height:
107%">Enzyme mix </span>

<span style="font-size:11.0pt;line-height:
107%">(vial1) </span>
| <span style="font-size:
11.0pt;line-height:107%">0.75ul </span>
| <span style="font-size:11.0pt;line-height:
107%">0.75ul </span>
| <span style="font-size:11.0pt;line-height:
107%">0.75ul </span>
| <span style="font-size:11.0pt;line-height:
107%"><span style="mso-spacerun:yes"> </span></span>
|-
| <span style="font-size:11.0pt;line-height:
107%">template </span>
| <span style="font-size:
11.0pt;line-height:107%">variable </span>
| <span style="font-size:11.0pt;line-height:
107%">variable </span>
| <span style="font-size:11.0pt;line-height:
107%">5ul </span>
|
<span style="font-size:11.0pt;line-height:
107%">1-50ng genomic DNA/ </span>

<span style="font-size:11.0pt;line-height:107%">10-100pg plasmid DNA </span>
|-
| '''<span style="font-size:11.0pt;line-height:107%">Total Volume </span>'''
| <span style="font-size:
11.0pt;line-height:107%">50ul </span>
| <span style="font-size:11.0pt;line-height:
107%">50ul </span>
| <span style="font-size:11.0pt;line-height:
107%">50ul </span>
| <span style="font-size:11.0pt;line-height:
107%"><span style="mso-spacerun:yes"> </span></span>
|}

<span style="line-height:150%">4)<span style="font:7.0pt "Times New Roman""> </span></span>Mix the reagents and centrifuge briefly to collect the sample at the bottom of the tube.

Note—Adjusting the DIG-dUTP concentration:

<span style="line-height:149%">•<span style="font:7.0pt "Times New Roman""> </span></span>The final concentration of DIG-dUTP (70uM), when using the undiluted PCR DIC probe Synthesis Mix (vial2), works well for labeling probes up to 1kb long.

<span style="line-height:149%">•<span style="font:7.0pt "Times New Roman""> </span></span>For labeling probes 1-3kb long, reduce the final concentration of DIG-dUTP to 35uM; Mix equal parts of PCR DIG probe synthesis Mix (vial 2) and dNTP stock solution (vial 4).

<span style="line-height:149%">•<span style="font:7.0pt "Times New Roman""> </span></span>For labeling short probes (<1Kb) of high GC content it might also be necessary to reduce the DIG-dUTP concentration to 35uM.

Ping’s note--

<span style="line-height:149%">•<span style="font:7.0pt "Times New Roman""> </span></span>For template, use water diluted cDNA mini prep product (25pg/ul). For above 50ml reaction, use 1 ul .

<span style="line-height:111%">•<span style="font:7.0pt "Times New Roman""> </span></span>Use (35uM DIG-dUTP) (2.5 ul of DIG probe mix (vial 2) + 2.5ul of dNTP (vial 4))

<span style="line-height:111%">5)<span style="font:7.0pt "Times New Roman""> </span></span>PCR program--

95°C 5min

95°C 30s<span style="font-family:Wingdings">à</span>55°C 30s <span style="font-family:Wingdings">à</span>72°C (time base on product) '''''10 cycles'''''

<span style="font-size:11.0pt;line-height:111%;font-family:"Calibri",sans-serif"> </span>95°C 30s<span style="font-family:Wingdings">à</span>55°C 30s<span style="font-family:Wingdings">à</span>72°C (time base on product) + 5s/cycle '''''20 cycles'''''

72°C 5min

8°C Forever

<span style="line-height:111%">6)<span style="font:7.0pt "Times New Roman""> </span></span>For PCR product, clean up with [https://www.zymoresearch.com/collections/dcc-pcr-purification-kits Zymo DNA Clean]. Check OD260.

<span style="line-height:111%">7)<span style="font:7.0pt "Times New Roman""> </span></span>Gel check

Run 2ul of labeled and unlabeled PCR onto 1% agarose gel to check the label (see picture below). If label is OK, use 40ul/20ml of labeled probe for screening.

<span style="font-size:11.0pt;line-height:107%;font-family:
"Calibri",sans-serif">[[Image:Digscreen_image1.png]]</span>

= 2. DIG high prime DNA label =

DNA is random primed labeled with Digoxigenin-11-dUTP using DIG-High Prime, a 5X concentrated labeling mixture of random hexamers, dNTP mix containing alkali-labile

Digoxigenin-11-dUTP, labeling grade Klenow enzyme and an optimized reaction buffer.

(For reagent and more details, please refer to [https://www.sigmaaldrich.com/US/en/product/roche/11745832910?gclid=Cj0KCQjwmvSoBhDOARIsAK6aV7g-Kc5DDj0UgH_5dn46wW_dMzd_uYX8rZ8nib91rY_1Ad9eeElC9gkaAuzfEALw_wcB DIG-High Prime DNA Labeling and Detection Starter Kit I].) *All solutions come from the Kit.

<span style="line-height:178%">1)<span style="font:7.0pt "Times New Roman""> </span></span>Add 1 μg '''template DNA''' (linear or supercoiled) and autoclaved, double distilled water to a final volume of 16 μl to a reaction vial.

<span style="line-height:149%">2)<span style="font:7.0pt "Times New Roman""> </span></span>Denature the DNA by heating in a boiling water bath for 10 min and quickly chilling in an ice/water bath.

'''''Note''''': Complete denaturation is essential for efficient labeling.

<span style="line-height:152%">3)<span style="font:7.0pt "Times New Roman""> </span></span>Mix '''DIG-High Prime''' (vial 1) thoroughly and add 4ul to the denatured DNA, mix and centrifuge briefly. Incubate for 1 h or O/N at 37°C.

'''''Note:''''' Longer incubations (up to 20 h) will increase the yield of DIG-labeled DNA.

<span style="line-height:154%">4)<span style="font:7.0pt "Times New Roman""> </span></span>Stop the reaction by adding 2ul 0.2 M '''EDTA''' (pH 8.0) and/or by heating to 65° C for 10 min.

'''''Note:''''' The lengths of the DIG labeled fragments obtained with DIG-High Prime range from 200 bp to 1000 bp or larger, depending on the lengths of the original template.

''' '''

= 3.<span style="font-weight:normal"> </span>Determination of labeling efficiency-Dilution and dot blot =

Determination of the yield of DIG-labeled DNA is most important for optimal and reproducible hybridization results. Too high of a probe concentration in the hybridization mix causes background, while too low of a concentration leads to weak signals.

(For reagent and more details, please refer to [https://www.sigmaaldrich.com/US/en/product/roche/11745832910?gclid=Cj0KCQjwmvSoBhDOARIsAK6aV7g-Kc5DDj0UgH_5dn46wW_dMzd_uYX8rZ8nib91rY_1Ad9eeElC9gkaAuzfEALw_wcB DIG-High Prime DNA Labeling and Detection Starter Kit I].) *All solutions come from the Kit.

<span style="line-height:152%">1)<span style="font:7.0pt "Times New Roman""> </span></span>Apply a 1μl spot of tubes 2-9 from your labeled probes and the labeled control to the nylon membrane.

<span style="line-height:149%">2)<span style="font:7.0pt "Times New Roman""> </span></span>Fix the nucleic acid to the membrane by cross linking with UV-light or baking for 30 min at 120°C.

<span style="line-height:152%">3)<span style="font:7.0pt "Times New Roman""> </span></span>Transfer the membrane into a plastic container with 20 ml '''Maleic acid buffer'''. Incubate under shaking for 2 min at 15-25°C.

<span style="line-height:111%">4)<span style="font:7.0pt "Times New Roman""> </span></span>Incubate for 30 min in 10 ml '''Blocking solution'''.

<span style="line-height:111%">5)<span style="font:7.0pt "Times New Roman""> </span></span>Incubate for 30 min in 10 ml '''Antibody solution.'''

<span style="line-height:111%">6)<span style="font:7.0pt "Times New Roman""> </span></span>Wash with 10 ml '''Washing buffer''', 2 × 15 min.

<span style="line-height:111%">7)<span style="font:7.0pt "Times New Roman""> </span></span>Equilibrate 2-5 min in 10 ml '''Detection buffer'''.

<span style="line-height:151%">8)<span style="font:7.0pt "Times New Roman""> </span></span>Place membrane with DNA side facing up on a development folder (or hybridization bag) and apply 0.1 ml '''CSPD ready-to-use''' (i.e. 4 drops from the dropper bottle 5) to the membrane.

'''Immediately''' cover the membrane with the second sheet of the folder to spread the substrate evenly and without air bubbles over the membrane. Incubate for 5 min at 15-25°C.

<span style="line-height:111%">9)<span style="font:7.0pt "Times New Roman""> </span></span>Squeeze out excess liquid and seal the edges of the development folder. '''''Note'':'''

Drying of the membrane during exposure will result in dark background.

<span style="line-height:149%">10)<span style="font:7.0pt "Times New Roman""> </span></span>Expose to an appropriate imager for 5-20 min or to X-ray film for 15-25 min at 1525°C.

'''''Note''''': Luminescence continues for at least 48 hours. The signal increases in the first few hours after initiation of the detection reaction until it will reach a plateau where signal intensity remains almost constant during the next 24 – 48 hours. Multiple exposures can be taken to achieve the desired signal strength.

<span style="font-size:11.0pt;line-height:107%;font-family:
"Calibri",sans-serif">[[Image:DIGscreen image2.png|410x262px]]</span>

Compare the intensity of the spots out of your labeling reaction to the control and calculate the amount of DIG-labeled DNA. If the 0.1 pg dilution spots of your probe and of the control are visible, then the labeled probe has reached the expected labeling efficiency. ''''''

= 4. Screen library =

''' '''

(For reagent and more details, please refer to [https://www.sigmaaldrich.com/US/en/product/roche/11745832910?gclid=Cj0KCQjwmvSoBhDOARIsAK6aV7g-Kc5DDj0UgH_5dn46wW_dMzd_uYX8rZ8nib91rY_1Ad9eeElC9gkaAuzfEALw_wcB DIG-High Prime DNA Labeling and Detection Starter Kit I].) *All solutions come from the Kit.

1) Stripping filters

('''''Important Tip: Membranes should never be allowed to dry before stripping.''''' ''Once dried, the membrane cannot be stripped and reprobed.'')

Stripping DIG-labeled DNA Probe after Chemiluminescent Detection

<span style="line-height:111%">•<span style="font:7.0pt "Times New Roman""> </span></span>Rinse membrane thoroughly with double distilled water for 1 min

<span style="line-height:150%">•<span style="font:7.0pt "Times New Roman""> </span></span>Wash membrane twice at 37°C in Stripping Buffer (0.2 M NaOH containing 0.1% SDS) for 15 min each.

<span style="line-height:111%">•<span style="font:7.0pt "Times New Roman""> </span></span>Rinse membrane with 2X SSC for 5 min

<span style="line-height:111%">2)<span style="font:7.0pt "Times New Roman""> </span></span>Prehybridization in 40ml DIG Easy Hyb at 48°C for 1 hour

<span style="line-height:149%">3)<span style="font:7.0pt "Times New Roman""> </span></span>Denature the probe--all probes mixed in 350ul 10uM Tris and denatured under 95°C for 5min, then chill quickly on ice bath

<span style="line-height:149%">4)<span style="font:7.0pt "Times New Roman""> </span></span>Hybridization solution-- Immediately add the denatured probe to a tube containing the appropriate amount of prewarmed DIG Easy Hyb (3.5 ml per 100 cm2) and

mix by inversion (avoid foaming) to form the hybridization solution

<span style="line-height:154%">5)<span style="font:7.0pt "Times New Roman""> </span></span>Pour off the prehybridization buffer.<span style="color:#F46E20"> </span>'''Immediately''' add hybridization solution containing DIG-labeled probe and hybridization at 48°C for 6-16 hours

<span style="line-height:150%">6)<span style="font:7.0pt "Times New Roman""> </span></span>Wash with low Stringency Buffer (1L 2X SSC containing 0.1% SDS) at room temperature for 15min twice

<span style="line-height:111%">7)<span style="font:7.0pt "Times New Roman""> </span></span>Preheat high Stringency Buffer (1L 0.5X SSC containing 0.1% SDS) into 65°C

<span style="line-height:149%">8)<span style="font:7.0pt "Times New Roman""> </span></span>Wash with preheated high Stringency Buffer (1L 0.5X SSC containing 0.1% SDS) at 65°C for 15min twice

= 5. Immunological Detection =

(Please refer to protocol of [https://www.sigmaaldrich.com/US/en/product/roche/11745832910?gclid=Cj0KCQjwmvSoBhDOARIsAK6aV7g-Kc5DDj0UgH_5dn46wW_dMzd_uYX8rZ8nib91rY_1Ad9eeElC9gkaAuzfEALw_wcB DIG-High Prime DNA Labeling and Detection Starter Kit I].) *All solutions come from the Kit.

<span style="line-height:149%">9)<span style="font:7.0pt "Times New Roman""> </span></span>After hybridization and stringency washes, rinse membrane for 2 min in '''Washing buffer'''.

<span style="line-height:111%">10)<span style="font:7.0pt "Times New Roman""> </span></span>Incubate in 250 ml 1X'''Blocking solution''' for 1 hour under room temperature

<span style="line-height:153%">11)<span style="font:7.0pt "Times New Roman""> </span></span>Prepare '''Antibody solution''': Spin AP antibody at 10rpm for 5min and then add AP antibody into 100ml 1Xblocking buffer into 1:10000.

<span style="line-height:151%">12)<span style="font:7.0pt "Times New Roman""> </span></span>Incubate in 100ml '''Antibody solution''' for 1 hour 13) Wash in 500ml '''Washing buffer''' (2X SSC)15min twice 14) Equilibrate 5 min in 20ml '''Detection buffer.'''

<span style="line-height:153%">15)<span style="font:7.0pt "Times New Roman""> </span></span>Incubate membrane in 10 ml freshly prepared '''color substrate solution''' in a appropriate container in the dark for 5 min. '''Do not shake''' during color development.

<span style="line-height:111%">16)<span style="font:7.0pt "Times New Roman""> </span></span>Expose 30min & develop film.

[[Image:DIGscreen image3.png]]<span style="font-size:11.0pt;line-height:
107%"> </span>

'''6. Check the correct probe for the positive clone'''

1) Pick up all positive clones and mini prep.

2) Put positive clones DNA to make dots in small filter

Redo the hybrization (steps 4(2) until the end) with individual probes to tell which probe this clone is responding to.

Resources

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Xenbase:

The combined resources provided by the preliminary Sea Urchin Genome Project are included on this site. The individual resources listed below are useful for gene discovery approaches, expressed sequence tag analysis and most importantly, studies of gene regulation in the sea urchin.

'''BAC Table'''<br />
BAC Table can be accessed here [[BAC_Table|BAC Table]]<br />
Please read following post to learn about our BACs resource and experimental methods<br />
[[recombac|Recombinant Bacs]]<br />
Annotation of BAC Sequences with Sea Urchin Gene AnnotatoR (SUGAR, no longer available)

'''BACs, BAC-ends and Gene Number'''<br />
A virtual map of the genome was constructed by sequencing the ends of 76,020 BAC recombinants (average length 125kb). The BAC-end sequence tag connectors (STCs) occur an average of 10kb apart. They can be used to assemble contigs surrounding any gene of interest. Using Blast matches to sequences from Bac-ends and complete BACs, confirmations from cDNA sequences we estimate that the sea urchin genome contains a total of 22+/-5X10<sup>3</sup> genes.

[[File:VirtualMap.gif|frame|left|Recovery of a contig surrounding a known gene by use of the STC database. (A) The arrayed BAC library is screened by using a cDNA probe, and a BAC containing the gene sequence is selected; the most desirable clone would be the longest that has the gene sequence (solid box) toward the middle. The STCs for that BAC (black-triangle, star) are recovered from the database together with the length distribution of restriction fragments produced by the sites (indicated by short, vertical lines). (B) Oligonucleotide probes are created from the left and right STCs and used to scan the BAC library. The selected clones are aligned to overlap in the restriction digest patterns. The probes should not lie within repetitive sequences: if they do, too many BACs will score positive, and their restriction fragment lengths will not align. (C) The clones extending the longest distance left and right plus the original clone (A) constitute a contig surrounding the gene, marked by STC tags at frequent intervals.]].

'''Repeats'''<br />
Since the first sea urchin genomic sequencing project was undertaken, a number of collections of the repeat sequences in the purple sea urchin have been collected. A survey of the 76,000 BAC ends for repeat sequences is described [http://www.ncbi.nlm.nih.gov/pubmed/10920195 here].

'''cDNA Libraries'''<br />
We maintain a suite of cDNA libraries from embryonic stages, larval stages and adult tissues of the reference species, Strongylocentrotus purpuratus. A few cDNA libraries from other species are also available [[filters|(see Table)]] These libraries are stored in 384-well plates permitting easy replication and re-spotting as needed. The libraries are spotted onto filters for screening. Library filters are available to members of the research community. [[Filters|(How to order)]]. These libraries have been used as the basis for EST sequencing as part of the original genome sequencing project reported in [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159423/?tool=pubmed Science.] Several other smaller EST projects have also been completed [http://dev.biologists.org/content/128/13/2615.long (for example: Zhu, X., Mahairas, G., Cameron, R. A., Davidson, E. H. and Ettensohn, C. A. Large-scale analysis of mRNAs expressed by primary mesenchyme cells of the sea urchin embryo. Development 128, 2615-2627, 2001)]. The clones sequenced in these projects are accessioned into Genbank and SpBase with the plate and well locations attached. Thus one can use sequence search programs to find the clones and then request them from our Resource. In other words "cloning by computer" is enabled here.

'''BAC Libraries'''<br />
Genomic sequence segments are maintained in bacterial artificial chromosome (BAC) libraries. We currently have libraries for seven species of lower deuterostomes in our resource [[filters|(see Table)]]The vector used is pBACe3.6 which originates from Children's Hospital Oakland Research Institute in Oakland, California, USA. This vector has chloramphenicol antibiotic resistance and is fully described in an article by [http://www.ncbi.nlm.nih.gov/pubmed/10373322?dopt=Abstract Frengen and colleages]. The vector sequence is available [http://www.ncbi.nlm.nih.gov/nuccore/4878025?report=fasta here]. The average insert size for our BAC libraries is about 140 Kb thus 1X coverage of the 800 Megabase genome of ''S. purpuratus'' is on average 5700 clones. Our libraries are at least 100,000 clones providing about 17X genome coverage.

'''cDNA sequences'''<br />
Approximately 13,000 cDNA sequences were obtained from the primary mesenchyme cell library. These sequences comprise 7,400 unique sequences when all of the overlaps are assembled. When these are searched against the BAC-end sequences, 1087 unique matches occur. Thus, the sequence matches between the BAC-ends, the ESTs, and the published data bases all give results commensurate with the conclusion that the collection of sequences we have obtained are of a quality suitable for gene discovery investigations in the sea urchin embryo. <span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=37498 (Cameron et al., Proc. Natl. Acad. Sci. USA, Vol. 97, Issue 17, 9514-9518, August 15, 2000 )]</span>

'''Gene Model'''<br />
During sea urchin genome project, several groups came up with gene predictions based on diverse approaches (ab initio, homology-based or empirical). Baylor used GLEAN methodology to combine those gene-sets into 28,944 unique genes. Their structures were derived from V0.5 genome assembly. At SpBase, we adopted Baylor's GLEAN genes and renamed each GLEAN IDs as GLEAN3_12345 to SPU_012345. New SPU genes will be added with IDs starting from 030000. This first release only modified the gene IDs from GLEAN and adopted them into SpBase. No real changes of gene structures were done.

'''Genome sequence traces'''<br />
Beginning in March 2003, the Baylor College of Medicine, Human Genome Sequencing center ([http://www.hgsc.bcm.tmc.edu www.hgsc.bcm.tmc.edu]) began to produce sea urchin sequences. First, a whole genome shotgun (WGS) project was undertaken and the individual sequences are deposited in the Genbank Trace Repository at [http://www.ncbi.nlm.nih.gov/Traces/home/ NCBI]. We have downloaded these traces,analyzed them by Blast and posted the matches in a searchable form on this web site. We will continue to do so until assembled genome sequences are posted at NCBI.

'''Quantitative PCR primers'''<br />
The Davidson laboratory at Caltech has generated a panel of quantitative PCR primers useful for measuring the level of mRNA abundance for genes involved in early development in general and the endomesoderm gene regulatory network in particular. A table of primer sequences and comments can be viewed ([[QPCR|here]]).

'''Nanostring Codeset'''<br />
The Nanostring nCounter identifies and counts RNA molecules based on a fluorescent barcode attached to a sequence specific hybridization ([http://www.ncbi.nlm.nih.gov/pubmed/?term=18278033 Geiss, G. K. et al,2008]). Our newly designed probe set contains codes for 341 genes covering the majority of active, and spatially restricted regulatory genes in the Strongylocentrotus purpuratus embryo up to pluteus (72 h post-fertilization). A description of the use of our previous code set was published by ([http://www.ncbi.nlm.nih.gov/pubmed/?term=20398801 Materna et al.]). They showed that Nanostring nCounter yields measurements with high fidelity over 5 orders of magnitude at levels down to a few transcripts per embryo. The genes and sequences in the new codeset are tabulated [[Nanostring|here]].

'''Sea Urchin Codon Usage Table'''<br />
The Sea Urchin codon usage table can be accessed [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=7668 here].

'''The principal investigators who participated in establishment of the Sea Urchin Genome Project:'''<br />
<p>Dr. Eric Davidson, California Institute of Technology</p>
<p>Dr. Charles Ettensohn, Carnegie-Mellon University</p>
<p>Dr. Leroy Hood, Institute for Systems Biology, Seattle Washington</p>
<p>Dr. Greg Wray Duke University, The Sea Urchin Genome Project</p>
<p>Dr. Andrew Cameron, California Institute of Technology</p>

'''Advisory Committee for the HGRI Sea Urchin Sequencing Project:'''<br />
<p>Eric H. Davidson, California Institute of Technology</p>
<p>R. Andrew Cameron, California Institute of Technology</p>
<p>Robert C. Angerer, National Institute of Dental and Craniofacial Research</p>
<p>Lynne Angerer, National Institute of Dental and Craniofacial Research</p>
<p>James A. Coffman, Mount Desert Island Biological Laboratory</p>
<p>William H. Klein, M. D. Anderson Cancer Center</p>
<p>Donal Manahan, University of Southern California</p>
<p>David R. McClay, Duke University</p>
<p>Jonathan P. Rast, University of Toronto</p>
<p>Victor D. Vacquier, Scripps Institute of Oceanography</p>

Filters

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Xenbase:

== Filters ==

The sea urchin genome research resource includes an extensive set of high-density arrayed cDNA and genomic libraries. These are carried permanently in 384-well plates and are reproduced for screening robotically, by spotting the clones in a format such that a 22 cm<sup>2</sup> membrane carries 18,432 clones in fixed locations with respect to both 384-well plate identity and X Y well location (Maier et al, 1994). Thus a whole cDNA library or large-fragment genomic library can be represented on five or six such filters. Each clone is spotted in duplicate to provide indicators for false positives. Each filter can<br />
be re used for screening many times, and after hybridization with a radioactive probe analysis of the filters can be carried out by digital methods. The large robot required for arraying and spotting clone libraries is housed at Caltech and thus we have continuous access to it.

The advantage of array technologies for the following proposals are crucial:

1. Instant availability of clones without purification by successive rescreening, i.e., when a high density screen reveals a desired clone as a pair of spots hybridizing with a given probe, it is at once available, pure, in a given position in a 384-well plate in the freezer.

2. Fast and easy mapping of extensive regions of the genome by use of overlaps in screens of BAC and PAC libraries. These libraries contain large genomic fragments. For example, in our ''S. purpuratus''
PAC library the average insert size is 80 kb; and in our BAC library it is 140 kb. The determination of the gene order in the ''Hox'' gene cluster of ''S. purpuratus'' performed by Martinez, P., Rast, J. P., Arenas-Mena, C.. and Davidson, E. H. <span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=37105 (Organization of an echinoderm ''Hox'' gene cluster. ''Proc. Natl. Acad. Sci. USA'' '''96''', 1469-1474, 1999.)]</span> illustrates how
such libraries can be exploited for genomic mapping.

3. Molecular techniques including subtractive hybridization methods and macro-array screening.

'''TABLE 1.''' The complement of arrayed libraries prepared as part of the Sea Urchin Genome Project. The progress in preparation of each library is indicated.
<br />
<br />
{|
| '''Source'''
| '''Made'''
| '''Arrayed'''
| '''Spotted'''
|-
| 72 hr Sp cDNA
| X
| X
| X<br />
|-
| Larval Sp cDNA
| X
| X
| X
|-
| PMC Sp cDNA
| X
| X
| X
|-
| Ovary Sp cDNA
| X
| X
| X
|-
| Testis Sp cDNA
| X
| X
| X
|-
| Am cDNA
| X
| X
| X
|-
| Blastula-Gastrula Ap cDNA
| X
| X
| X
|-
| Sp BAC genomic
| X
| X
| X
|-
| Sp small BAC
| X
| X
| X
|-
| Sp BAC FR2
| X
| X
| X
|-
| Lv BAC
| X
| X
| X
|-
| Et small BAC
| X
| X
| X
|-
| Lv small BAC
| X
| X
| X
|-
| Pl small BAC
| X
| X
| X
|-
| Am small BAC
| X
| X
| X
|-
| Ap BAC
| X
| X
| X
|-
| Pf BAC
| X
| X
| X
|-
| Sp BAC(Mbo1)
| X
| X
| X
|-
| Am large BAC
| X
| X
| X
|}

'''Abbreviations:'''

Sp: ''Strongylocentrotus purpuratus''<br/>
Sf: ''Strongylocentrotus franciscanus''<br/>
Lv: ''Lytechinus variegatus''<br/>
Ap: ''Arbacia punctulata''<br/>
Et: ''Eucidaris tribuloides''<br/>
Am: ''Asterina miniata''<br/>
Pf: ''Ptychodera flava''<br/>
Pl: ''Paracentrotus lividis''<br/>
PMC: ''primary mesenchyme cell''<br/>

== Ordering Filters ==

The arrayed filters are available to research workers at academic institutions.Please send a descriptive email telling us which filters you want and the purpose for which you intend to use them. Include a complete FEDEX shipping address with street address and phone numbers. Also give us your FEDEX account number to pay the shipping. The cost for filter sets is $600 each. We will send a letter with the order that will act as an invoice for payment. There is a short materials and methods on the website (here) that will tell you how to use them. As you find clones that you wish to request we expect to be told the probe used, etc. Later when you have analysed the clones we expect information about sequence, gene identity, etc. This information is placed in a database that is accessible on the website. In this manner we make the filters more valuable to the users.

Libraries, Vectors and Screening

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<p>Below is a list of links to common methods. If you would like to add to the list please contact us.</p>

=== An index of general molecular biology techniques used in studies of ''S. purpuratus'' ===

[[array|Arrayed Filter Manual]]<br />
The techniques used to hybridize probes to arrays of cDNAs on filters are detailed here. The manual is based on pages from the RZPD with modifications installed at Caltech.

[[download/DIGscreen.pdf|Non-radioactive library screening protocol]]<br />
The availability of sensitive chemiluminescent detection systems for hybridization screening of arrayed library filters now makes it possible to reach outcomes similar to methods using 32P labeled probes. We have used a digoxygenin based protocol with good success. It is detailed [[download/DIGscreen.pdf|here]].

[[subtrmethods|Subtractive Probe Analysis]]<br />
Our techniques for complex probes to be used on arrayed filters for gene discovery have been perfected by Jonathan Rast in the Davidson Laboratory. The investigation underlying this work is published: Rast J. P. et al, Developmental Biology 228 (2): 270-286, 2000. Supplementary data is presented here, as well.

[[libprepd|Additional Notes on cDNA Library Preparations]]<br />
The procedures used to construct the cDNA libraries are described here.

[[epgfp|The Reporter Plasmid, EpGFPII]]<br />
The sequence and map of the reporter construct described in: R. A. Cameron, P. Oliveri, J. Wyllie, and E. H. Davidson (2004) cis-Regulatory Activity of Randomly Chosen Genomic Fragments from the Sea Urchin. Gene Expression Patterns 4, 205-213 can be found here.

[[bac_vector_library|BAC Library Vector]]<br />
The BAC vector pBACe3.6 is the one we have used in the preparation of all of our macro-array genomic libraries. Constructed in Pieter J. de Jong's laboratory at the Children's Hospital Oakland Research Center, it has been used extensively for genomic libraries and sequencing projects of many species.

<span class="newwin">Fluorescent Protein Vectors</span><br />
The expression of exogenous proteins in sea urchins embryos, by injection of recombinant mRNAs, is routine procedure. The Hamdoun lab has generated a vector set with the major color and tag position variants that is useful for fluorescent protein tagging and expression in urchins. The complete vector set and can be obtained here: [https://www.addgene.org/kits/hamdoun-fluorescent-proteins/]. A description of the vectors can be found in Gokirmak et al., 2012 [http://www.echinobase.org/literature/article.do?method=display&articleId=42631 Vectors for generation of exogenous mRNAs fused to fluorescent proteins for expression in urchins]

BAC resources

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Xenbase:

== Genomic BAC Resources ==

Echinobase stores libraries of genomic sequence fragments maintained in bacterial artificial chromosome (BAC) vectors for seven lower deuterostome species [[BAC_Table|BAC Table]]. These libraries were originally constructed as part of the Sea Urchin Genome Project. On average, the insert sizes for our BAC libraries are ~140 kb. Thus, for the 800 Mb ''S. purpuratus'' genome, 1X coverage is encompassed by, on average, 5,700 clones. Each library consists of >100,000 clones, providing ~17X genome coverage.

A subset of the ''S. purpuratus'' BACs have been mapped to the genome assembly. As part of the SUGP, about 8,000 BAC clones were end sequenced using Sanger technology. Genomic positions for these BACs are indicated as a track in JBrowse (link). An additional set of individual BACs were sequenced completely; links to the NCBI page is provided for these. Finally, some BACs have been identified using hybridization screening of arrayed library filters (see [[BAC_Table|BAC Table ]]).

'''BAC Vector Sequence'''. The BACs are maintained in the [https://bacpacresources.org/pbace36.htm pBACe3.6 vector] which originates from Children's Hospital Oakland Research Institute in Oakland, California, USA. This vector confers resistance to the antibiotic chloramphenicol and is fully described by <span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=48450 Frengen and colleages]</span>. The vector sequence is available [http://www.ncbi.nlm.nih.gov/nuccore/4878025?report=fasta here].

'''Relevant publications:'''<br />
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=37498 A sea urchin genome project: Sequence scan, virtual map, and additional resources. Cameron, RA, et al., 2000. Proc. Natl. Acad. Sci. USA.]</span><br />
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=45819 Bacterial artificial chromosomes as recombinant reporter constructs to investigate gene expression and regulation in echinoderms. Buckley, KM et al., 2017. Briefings in Functional Genomics]</span>.

== BAC Protocols ==

Screening a BAC library<br />
Generating a recombinant BAC<br />
BAC miniprep protocol<br />
Analyzing BAC DNA by PFGE

<br />

== Recombinant BAC Resources ==

Echinobase has generated and maintains a set of reporter BAC constructs in which a portion of the gene of interest is replaced by the sequence encoding a fluorescent protein (''i.e.'' GFP, mCherry). A list of these sequences is available [[BAC_Table|here]]). Existing clones are available by request. We will also consider requests to make additional recombinant reporter constructs as time permits.

'''To request a recombinant or wildtype BAC for non-commercial use:'''<br />
1. Identify the BAC of interest computationally or by screening our libraries ([[filters]]).<br />
2. Request the BAC by emailing [mailto:ctelmer@cmu.edu Cheryl Telmer] or <span class="newwin">[http://www.echinobase.org/community/person.do?method=display&personId=4136&tabId=0 Veronica Hinman]</span>.

<br />

[[File:Bac.jpg|600px]]

== Working with BACs ==

'''Storing BACs'''

'''Short term'''<br />
To avoid shearing the long BAC DNA fragments, ''isolated BACs should never be vortexed or frozen''. Store BAC DNA at 4 C in TE (10 mM Tris, pH = 7.4; 10 mM EDTA).<br />
'''Long term storage'''<br />
Store glycerol bacteria stocks (15-25% glycerol) at -80 C.

'''Antibiotic conditions for working with BAC clones'''<br />
'''Kanamycin'''<br />
Stock (25 μg/μL): Dissolve 0.5 g of kanamycin into 10 ml of ddH2O. Filter through a 0.22 µm filter to sterilize. Aliquot and store at -20°C.<br />
Use at 1:1000 dilution (25 μg/mL).<br />
'''Chloramphenicol'''<br />
Stock (25 μg/μl): Dissolve in ETOH store at -20.<br />
Use at 1:2000 dilution.(12.5 μg/ml)

Epgfp

2023-10-06T15:01:28Z

Xenbase: /* EpGFPII */

<p>The plasmid vector used as a vehicle to test the cis-regulatory function of randomly cloned genomic DNA fragments was modified from a previous construct, EpGFP (Arnone et al., 1997; Arnone et al., 1998). The grand-parent of this plasmid was pCMV (Clontech); it has ampicillin resistance. EpGFP contains the region around the start of transcription of the endo16 gene (from -117 to +20).The activity of this element has been described in detail elsewhere (Yuh and Davidson, 1996; Yuh et al., 1996,1998, 2001). In addition to a TATA-containing basal promoter, it contains auxiliary sites for a ubiquitous sea urchin transcription factor which promotes DNA looping and has weak transcriptional enhancement activity(GCF1; Zeller et al., 1995). The vector also includes a sea urchin ribosome binding site from the cyIIa gene (Arnone et al., 1998), positioned at the start of the coding sequence of the [http://www.fpbase.org/protein/gfp-s65t/ GFP (S65T)] reporter. The original EpGFP construct was further modified by the addition of a double-stranded oligonucleotide polylinker inserted between the MluI and XmaI sites in the Multiple Cloning Site (MCS), so that the MCS contains the following restriction enzyme sites: 5'-KpnI-SacI-MluI-EcoRI-SpeI-BglII-SmaI/XmaI-3', situated just upstream of the endo16 basal promoter.</p>

== EpGFPII ==

[[image:Small_map.jpg||left|700x700px|EpGFPII plasmid vector]]

<div class="toccolours mw-collapsible mw-collapsed" style="clear:both">
EpGFPII FASTA Sequence
<div class="mw-collapsible-content">>epgfpii
<pre>
1 11 21 31 41 51 61 71 81 90
| | | | | | | | | |
GGTACCGAGC TCTTACGCGT GAATTCACTA GTAGATCTCC CGGGTTAAAC TGTTTGAGTT TCGTCTCCTG ATTGTGCTAT CAAAGACAAA 90 epgfpii
GGGGTGTAAC TTTACCCCCC TCATCAAGAG CGGAGGGTTA AATAGAGAAA GACTGGTCGA GGACAGGTCA TAATATTGCT AATTTTTAAG 180 epgfpii
CTTATCATCA TGTGTGACGA CGACGTCGCC GCTCTTGTCG TCGACAACGG ATCTGCGGCC GCCGCCACCA TGAGCAAGGG CGAGGAACTG 270 epgfpii
TTCACTGGCG TGGTCCCAAT TCTCGTGGAA CTGGATGGCG ATGTGAATGG GCACAAATTT TCTGTCAGCG GAGAGGGTGA AGGTGATGCC 360 epgfpii
ACATACGGAA AGCTCACCCT GAAATTCATC TGCACCACTG GAAAGCTCCC TGTGCCATGG CCAACACTGG TCACTACCTT CACCTATGGC 450 epgfpii
GTGCAGTGCT TTTCCAGATA CCCAGACCAT ATGAAGCAGC ATGACTTTTT CAAGAGCGCC ATGCCCGAGG GCTATGTGCA GGAGAGAACC 540 epgfpii
ATCTTTTTCA AAGATGACGG GAACTACAAG ACCCGCGCTG AAGTCAAGTT CGAAGGTGAC ACCCTGGTGA ATAGAATCGA GCTGAAGGGC 630 epgfpii
ATTGACTTTA AGGAGGATGG AAACATTCTC GGCCACAAGC TGGAATACAA CTATAACTCC CACAATGTGT ACATCATGGC CGACAAGCAA 720 epgfpii
AAGAATGGCA TCAAGGTCAA CTTCAAGATC AGACACAACA TTGAGGATGG ATCCGTGCAG CTGGCCGACC ATTATCAACA GAACACTCCA 810 epgfpii
ATCGGCGACG GCCCTGTGCT CCTCCCAGAC AACCATTACC TGTCCACCCA GTCTGCCCTG TCTAAAGATC CCAACGAAAA GAGAGACCAC 900 epgfpii
ATGGTCCTGC TGGAGTTTGT GACCGCTGCT GGGATCACAC ATGGCATGGA CGAGCTGTAC AAGTGAGCGG CCGCGGCTCG AGGCTCTAGA 990 epgfpii
GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 1080 epgfpii
TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA 1170 epgfpii
TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG 1260 epgfpii
ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC 1350 epgfpii
ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 1440 epgfpii
ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 1530 epgfpii
CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1620 epgfpii
GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1710 epgfpii
CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1800 epgfpii
CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1890 epgfpii
ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1980 epgfpii
GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 2070 epgfpii
ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 2160 epgfpii
TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 2250 epgfpii
CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 2340 epgfpii
TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 2430 epgfpii
ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2520 epgfpii
CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 2610 epgfpii
TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2700 epgfpii
ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 2790 epgfpii
AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2880 epgfpii
AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 2970 epgfpii
TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 3060 epgfpii
TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 3150 epgfpii
AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 3240 epgfpii
ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG 3330 epgfpii
CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 3420 epgfpii
TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 3510 epgfpii
CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 3600 epgfpii
GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 3690 epgfpii
TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT CCCATTCGCC 3780 epgfpii
ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCCCAAGCTA CCATGATAAG TAAGTAATAT 3870 epgfpii
TAAGGTACGG GAGGTACTTG GAGCGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA 3960 epgfpii
CTAACATACG CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT 4050 epgfpii
CTCTATCGAT A 4061 epgfpii
</pre></div>
</div>
[http://ftp.echinobase.org/pub/WikiDownloads/epgfpii.fasta Download FASTA file for EpGFPII]

=== REFERENCES ===

Yuh, C. H., Moore, J. G. and Davidson, E. H. Quantitative functional interrelations within the cis-regulatory system of the S. purpuratus Endo16 gene. Development 122, 4045-4056, 1996.<span class="newwin">[https://www.echinobase.org/literature/article.do?method=display&articleId=36543 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H. and Davidson, E. H. Modular cis-regulatory organization of Endo16, a gut-specific gene of the sea urchin embryo. Development 122, 1069-1082, 1996.<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36421 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H., Bolouri, H. and Davidson, E. H. Genomic cis-regulatory logic: Functional analysis and computational model of a sea urchin gene control system. Science 279, 1896-1902, 1998.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36860 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H., Bolouri, H. and Davidson, E. H. cis-Regulatory logic in the endo16 gene: Switching from a specification to a differentiation mode of control. Development 128, 617-628, 2001.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=37679 [open<nowiki>]</nowiki>]</span>

Zeller, R. W., Coffman, J. A., Harrington, M. G., Britten, R. J. and Davidson, E. H. SpGCF1, a sea urchin embryo transcription factor, exists as five nested variants encoded by a single mRNA. Dev. Biol. 169, 713-727, 1995.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=35795 [open<nowiki>]</nowiki>]</span>

Arnone, M. and Davidson, E. H. The hardwiring of development: Organization and function of genomic regulatory systems. Development 124, 1851-1864, 1997.

Arnone, M. I., Martin, E. L. and Davidson. E. H. Cis-regulation downstream of cell type specification: A single compact element controls the complex expression of the CyIIa gene in sea urchin embryos. Development 125, 1381-1395, 1998.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36858 [open<nowiki>]</nowiki>]</span>

Epgfp

2023-10-06T15:00:41Z

Xenbase:

<p>The plasmid vector used as a vehicle to test the cis-regulatory function of randomly cloned genomic DNA fragments was modified from a previous construct, EpGFP (Arnone et al., 1997; Arnone et al., 1998). The grand-parent of this plasmid was pCMV (Clontech); it has ampicillin resistance. EpGFP contains the region around the start of transcription of the endo16 gene (from -117 to +20).The activity of this element has been described in detail elsewhere (Yuh and Davidson, 1996; Yuh et al., 1996,1998, 2001). In addition to a TATA-containing basal promoter, it contains auxiliary sites for a ubiquitous sea urchin transcription factor which promotes DNA looping and has weak transcriptional enhancement activity(GCF1; Zeller et al., 1995). The vector also includes a sea urchin ribosome binding site from the cyIIa gene (Arnone et al., 1998), positioned at the start of the coding sequence of the [http://www.fpbase.org/protein/gfp-s65t/ GFP (S65T)] reporter. The original EpGFP construct was further modified by the addition of a double-stranded oligonucleotide polylinker inserted between the MluI and XmaI sites in the Multiple Cloning Site (MCS), so that the MCS contains the following restriction enzyme sites: 5'-KpnI-SacI-MluI-EcoRI-SpeI-BglII-SmaI/XmaI-3', situated just upstream of the endo16 basal promoter.</p>

== EpGFPII ==

[[image:Small_map.jpg||left|700x700px|EpGFPII plasmid vector]]

<div class="toccolours mw-collapsible mw-collapsed" style="clear:both">
EpGFPII FASTA Sequence
<div class="mw-collapsible-content">>epgfpii
<pre>
1 11 21 31 41 51 61 71 81 90
| | | | | | | | | |
GGTACCGAGC TCTTACGCGT GAATTCACTA GTAGATCTCC CGGGTTAAAC TGTTTGAGTT TCGTCTCCTG ATTGTGCTAT CAAAGACAAA 90 epgfpii
GGGGTGTAAC TTTACCCCCC TCATCAAGAG CGGAGGGTTA AATAGAGAAA GACTGGTCGA GGACAGGTCA TAATATTGCT AATTTTTAAG 180 epgfpii
CTTATCATCA TGTGTGACGA CGACGTCGCC GCTCTTGTCG TCGACAACGG ATCTGCGGCC GCCGCCACCA TGAGCAAGGG CGAGGAACTG 270 epgfpii
TTCACTGGCG TGGTCCCAAT TCTCGTGGAA CTGGATGGCG ATGTGAATGG GCACAAATTT TCTGTCAGCG GAGAGGGTGA AGGTGATGCC 360 epgfpii
ACATACGGAA AGCTCACCCT GAAATTCATC TGCACCACTG GAAAGCTCCC TGTGCCATGG CCAACACTGG TCACTACCTT CACCTATGGC 450 epgfpii
GTGCAGTGCT TTTCCAGATA CCCAGACCAT ATGAAGCAGC ATGACTTTTT CAAGAGCGCC ATGCCCGAGG GCTATGTGCA GGAGAGAACC 540 epgfpii
ATCTTTTTCA AAGATGACGG GAACTACAAG ACCCGCGCTG AAGTCAAGTT CGAAGGTGAC ACCCTGGTGA ATAGAATCGA GCTGAAGGGC 630 epgfpii
ATTGACTTTA AGGAGGATGG AAACATTCTC GGCCACAAGC TGGAATACAA CTATAACTCC CACAATGTGT ACATCATGGC CGACAAGCAA 720 epgfpii
AAGAATGGCA TCAAGGTCAA CTTCAAGATC AGACACAACA TTGAGGATGG ATCCGTGCAG CTGGCCGACC ATTATCAACA GAACACTCCA 810 epgfpii
ATCGGCGACG GCCCTGTGCT CCTCCCAGAC AACCATTACC TGTCCACCCA GTCTGCCCTG TCTAAAGATC CCAACGAAAA GAGAGACCAC 900 epgfpii
ATGGTCCTGC TGGAGTTTGT GACCGCTGCT GGGATCACAC ATGGCATGGA CGAGCTGTAC AAGTGAGCGG CCGCGGCTCG AGGCTCTAGA 990 epgfpii
GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 1080 epgfpii
TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA 1170 epgfpii
TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG 1260 epgfpii
ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC 1350 epgfpii
ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 1440 epgfpii
ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 1530 epgfpii
CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1620 epgfpii
GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1710 epgfpii
CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1800 epgfpii
CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1890 epgfpii
ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1980 epgfpii
GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 2070 epgfpii
ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 2160 epgfpii
TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 2250 epgfpii
CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 2340 epgfpii
TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 2430 epgfpii
ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2520 epgfpii
CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 2610 epgfpii
TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2700 epgfpii
ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 2790 epgfpii
AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2880 epgfpii
AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 2970 epgfpii
TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 3060 epgfpii
TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 3150 epgfpii
AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 3240 epgfpii
ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG 3330 epgfpii
CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 3420 epgfpii
TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 3510 epgfpii
CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 3600 epgfpii
GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 3690 epgfpii
TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT CCCATTCGCC 3780 epgfpii
ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCCCAAGCTA CCATGATAAG TAAGTAATAT 3870 epgfpii
TAAGGTACGG GAGGTACTTG GAGCGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA 3960 epgfpii
CTAACATACG CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT 4050 epgfpii
CTCTATCGAT A 4061 epgfpii
</pre></div>
</div>
[http://ftp.echinobase.org/pub/WikiDownloads/epgfpii.fasta Download FASTA file for EpGFPII]

=== REFERENCES ===

Yuh, C. H., Moore, J. G. and Davidson, E. H. Quantitative functional interrelations within the cis-regulatory system of the S. purpuratus Endo16 gene. Development 122, 4045-4056, 1996.<span class="newwin">[https://www.echinobase.org/literature/article.do?method=display&articleId=36543 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H. and Davidson, E. H. Modular cis-regulatory organization of Endo16, a gut-specific gene of the sea urchin embryo. Development 122, 1069-1082, 1996.<span class="newwin">[http://new.echinobase.org/literature/article.do?method=display&articleId=36421 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H., Bolouri, H. and Davidson, E. H. Genomic cis-regulatory logic: Functional analysis and computational model of a sea urchin gene control system. Science 279, 1896-1902, 1998.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36860 [open<nowiki>]</nowiki>]</span>

Yuh, C.-H., Bolouri, H. and Davidson, E. H. cis-Regulatory logic in the endo16 gene: Switching from a specification to a differentiation mode of control. Development 128, 617-628, 2001.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=37679 [open<nowiki>]</nowiki>]</span>

Zeller, R. W., Coffman, J. A., Harrington, M. G., Britten, R. J. and Davidson, E. H. SpGCF1, a sea urchin embryo transcription factor, exists as five nested variants encoded by a single mRNA. Dev. Biol. 169, 713-727, 1995.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=35795 [open<nowiki>]</nowiki>]</span>

Arnone, M. and Davidson, E. H. The hardwiring of development: Organization and function of genomic regulatory systems. Development 124, 1851-1864, 1997.

Arnone, M. I., Martin, E. L. and Davidson. E. H. Cis-regulation downstream of cell type specification: A single compact element controls the complex expression of the CyIIa gene in sea urchin embryos. Development 125, 1381-1395, 1998.
<span class="newwin">[http://www.echinobase.org/literature/article.do?method=display&articleId=36858 [open<nowiki>]</nowiki>]</span>

File:Bac.jpg

2023-10-04T18:42:37Z

Xenbase: Xenbase uploaded File:Bac.jpg

BAC Table

2023-05-15T15:00:55Z

Xenbase:

<div id="main-inner" class="clearfix" style="width: 900px;">

== BAC Table ==

Sequences of BACs (as best we know them) can be accessed by clicking on BAC NCBI link.

These BAC clones are available by request. We will also entertain requests to make additional constructs as time permits. For new preparations, you will need to find the BAC by computer or by screening our libraries. In order to prepare a new recombinant BAC we will need the ID of the BAC clone from our libraries, the sequence of the gene it contains and evidence that you know the position of the gene in the BAC. This is a bit complicated to explain here.

To order BACs for non-commercial purpose please contact [https://www.echinobase.org/entry/community/person.do?personId=4136&method=displayProfile Veronica Hinman].
Here is a brief [https://wiki.echinobase.org/echinowiki/index.php/Echinobase/bac_information BAC Library Guide].

{| style="border:solid 1px black" class="sortable wikitable"
!Species!!Gene Name!!BAC Name!!Reporter!!NCBI Link!!BAC Size!!WHL ID!!SPU ID!!Comment
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

3Acolf/3Apcol

</div>
|
<div class="style8" align="left">

Sp_3055B12

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

125kb

</div>
|
<div class="style8" align="left">

WHL22.438943

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

3Apcol(W01)

</div>
|
<div class="style8" align="left">

Sp_3066N3

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

125kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

3Apcol(w88)

</div>
|
<div class="style8" align="left">

Sp_R33066N3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.589988

</div>
|
<div class="style8" align="left">

SPU_003768

</div>
|
<div class="style8" align="left">

Echinobase length:122kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

3'Hex

</div>
|
<div class="style8" align="left">

Sp_R33044F0

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC176398.1 AC176398.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_021829

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Acsbg1

</div>
|
<div class="style8" align="left">

Sp_1020M3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.59646,WHL22.59649

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ada_1

</div>
|
<div class="style8" align="left">

Sp_113F15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ada_1

</div>
|
<div class="style8" align="left">

Sp_R3113F15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.162939.1

</div>
|
<div class="style8" align="left">

SPU_023134

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Akt

</div>
|
<div class="style8" align="left">

Sp_3050P16

</div>
|
<div class="style8" align="left">

Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Alx1

</div>
|
<div class="style8" align="left">

Sp_143J11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Alx1

</div>
|
<div class="style8" align="left">

Sp_042I08_L|Sp_42I8

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

133kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Alx1_1

</div>
|
<div class="style8" align="left">

Sp_R33063K17

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

135kb

</div>
|
<div class="style8" align="left">

WHL22.731056

</div>
|
<div class="style8" align="left">

SPU_025302

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ankfy12

</div>
|
<div class="style8" align="left">

Sp_3097C6

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

50kb ,100kb

</div>
|
<div class="style8" align="left">

WHL.22.751898

</div>
|
<div class="style8" align="left">

SPU_024172

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Apobec

</div>
|
<div class="style8" align="left">

Sp_112F15_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC130962 AC130962]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Arhgap28

</div>
|
<div class="style8" align="left">

Sp_3080H22

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

80kb,200kb

</div>
|
<div class="style8" align="left">

WHL.22.409669

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

Echinobase length:155kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Arhgap28

</div>
|
<div class="style8" align="left">

Sp_R33097N6

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

80kb,200kb

</div>
|
<div class="style8" align="left">

WHL22.409669

</div>
|
<div class="style8" align="left">

SPU_027628

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Astacin1

</div>
|
<div class="style8" align="left">

Sp_3004P19

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Astacin1

</div>
|
<div class="style8" align="left">

Sp_R33088G04

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

125kb,160kb

</div>
|
<div class="style8" align="left">

WHL22.642085

</div>
|
<div class="style8" align="left">

SPU_019655

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Astacin5

</div>
|
<div class="style8" align="left">

Sp_17G24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

125kb,160kb

</div>
|
<div class="style8" align="left">

WHL.22.642085.0

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Astacin5

</div>
|
<div class="style8" align="left">

Sp_R34007P18

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

125kb,160kb

</div>
|
<div class="style8" align="left">

WHL22.237823

</div>
|
<div class="style8" align="left">

SPU_002178

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Atbf1

</div>
|
<div class="style8" align="left">

Sp_3042J1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

156kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

B4galt6_8

</div>
|
<div class="style8" align="left">

Sp_R33045N1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.385964

</div>
|
<div class="style8" align="left">

SPU_007019

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Betacatenin

</div>
|
<div class="style8" align="left">

Sp_154N12_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131447 AC131447]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Blimp1

</div>
|
<div class="style8" align="left">

Sp_163O19_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131508 AC131508]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Bmp2/4

</div>
|
<div class="style8" align="left">

Sp_1108H16

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Brachyury

</div>
|
<div class="style8" align="left">

Sp_117A03_L

</div>
|
<div class="style8" align="left">

GFP,RFP,Dendra

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131378 AC131378]

</div>
|
<div class="style8" align="left">

131kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Brn 124

</div>
|
<div class="style8" align="left">

Sp_16C24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

207kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Brn 124

</div>
|
<div class="style8" align="left">

Sp_180F15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Brn 124

</div>
|
<div class="style8" align="left">

Sp_95O3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

C14orf124

</div>
|
<div class="style8" align="left">

Sp_R33083P17

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.38

</div>
|
<div class="style8" align="left">

SPU_027030

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

C3bf

</div>
|
<div class="style8" align="left">

Sp_076N15_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

C4mso

</div>
|
<div class="style8" align="left">

Sp_3002L17

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=MK371211 MK371211]

</div>
|
<div class="style8" align="left">

110kb

</div>
|
<div class="style8" align="left">

WHL.22.317555.0.,WHL.22.317555 .1

</div>
|
<div class="style8" align="left">

SPU_018974

</div>
|
<div class="style8" align="left">

Echinobase length:33kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

C6orf70L

</div>
|
<div class="style8" align="left">

Sp_14B10,Sp_3101E10,Sp_3099N11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

C6orf70L

</div>
|
<div class="style8" align="left">

Sp_R34013G18

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.440137

</div>
|
<div class="style8" align="left">

SPU_020361

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Cah10L

</div>
|
<div class="style8" align="left">

Sp_R321I13

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Cah10L

</div>
|
<div class="style8" align="left">

Sp_R33055A16

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.642603

</div>
|
<div class="style8" align="left">

SPU_004135

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Cah10L

</div>
|
<div class="style8" align="left">

Sp_R33057B15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.642603

</div>
|
<div class="style8" align="left">

SPU_004136

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Capk

</div>
|
<div class="style8" align="left">

Sp_083N11_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131512 AC131512]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

CcLta1

</div>
|
<div class="style8" align="left">

Sp_R38H4

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

150000

</div>
|
<div class="style8" align="left">

WHL22.764666

</div>
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<div class="style8" align="left">

SPU_011692

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Cdx

</div>
|
<div class="style8" align="left">

Sp_3088G4

</div>
|
<div class="style8" align="left">

Cherry, GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Clect_14

</div>
|
<div class="style8" align="left">

Sp_3110K19

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Clect_14

</div>
|
<div class="style8" align="left">

Sp_R33032K03

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.39473

</div>
|
<div class="style8" align="left">

SPU_005991

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

ClvhH1

</div>
|
<div class="style8" align="left">

Sp_R33103O23

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

11kb

</div>
|
<div class="style8" align="left">

WHL22.674821

</div>
|
<div class="style8" align="left">

SPU_024567

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Col4a2

</div>
|
<div class="style8" align="left">

Sp_R33077D06

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.77073

</div>
|
<div class="style8" align="left">

SPU_015708

</div>
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<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Cpa2L_3

</div>
|
<div class="style8" align="left">

Sp_R31040I20

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.153705

</div>
|
<div class="style8" align="left">

SPU_014935

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Csrnp2

</div>
|
<div class="style8" align="left">

Sp_43E11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

155kb

</div>
|
<div class="style8" align="left">

WHL22.749825.0 /1

</div>
|
<div class="style8" align="left">

SPU_025496

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Cyclophillin

</div>
|
<div class="style8" align="left">

Sp_014A23_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Cycpln

</div>
|
<div class="style8" align="left">

1111 B19

</div>
|
<div class="style8" align="left">

(No GFP)

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.733408

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dach

</div>
|
<div class="style8" align="left">

Sp_12 P15

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

246kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dachouslik

</div>
|
<div class="style8" align="left">

Sp_R33025P1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC178568.1 AC178568.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_008299

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Deaf1L

</div>
|
<div class="style8" align="left">

Sp_R34B03

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.158312

</div>
|
<div class="style8" align="left">

SPU_022541

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Decorin

</div>
|
<div class="style8" align="left">

Sp_119C23_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Delta

</div>
|
<div class="style8" align="left">

Sp_46A16

</div>
|
<div class="style8" align="left">

Cherry,GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131451 AC131451]

</div>
|
<div class="style8" align="left">

126kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Derp13

</div>
|
<div class="style8" align="left">

Sp_R34010K23

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

120kb

</div>
|
<div class="style8" align="left">

WHL22.505560

</div>
|
<div class="style8" align="left">

SPU_007210

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dlx

</div>
|
<div class="style8" align="left">

Sp_26L24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dlx

</div>
|
<div class="style8" align="left">

Sp_47I19

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dopt

</div>
|
<div class="style8" align="left">

Sp_188B16_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131448 AC131448]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dr

</div>
|
<div class="style8" align="left">

Sp_44J22

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=MK371208 MK371208]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.544154

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dr

</div>
|
<div class="style8" align="left">

Sp_R316F8

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.544154

</div>
|
<div class="style8" align="left">

SPU_017106

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dri

</div>
|
<div class="style8" align="left">

Sp_118J14_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

135kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Dri

</div>
|
<div class="style8" align="left">

Sp_74F08

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

142kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

E2f3

</div>
|
<div class="style8" align="left">

Sp_3093P10

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

17kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

E2f3

</div>
|
<div class="style8" align="left">

Sp_3100O17

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ecr (fxr)

</div>
|
<div class="style8" align="left">

Sp_1018H13

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Elk

</div>
|
<div class="style8" align="left">

Lv_12B5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

182kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Elk

</div>
|
<div class="style8" align="left">

Sp_23B5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Endo16

</div>
|
<div class="style8" align="left">

Sp_127I21_S

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC160518 AC160518]

</div>
|
<div class="style8" align="left">

55kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ephrin

</div>
|
<div class="style8" align="left">

Sp_024N08_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Erg

</div>
|
<div class="style8" align="left">

Sp_14H16

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

151kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Erg

</div>
|
<div class="style8" align="left">

Sp_R33063M23

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.552472

</div>
|
<div class="style8" align="left">

SPU_018483

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Erg

</div>
|
<div class="style8" align="left">

Sp_R33085K1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC178220.1 AC178220.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_009100

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ese

</div>
|
<div class="style8" align="left">

Sp_1032O14

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ese

</div>
|
<div class="style8" align="left">

Sp_3107I24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

163kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ets1

</div>
|
<div class="style8" align="left">

Sp_28J16

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

110kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ets1

</div>
|
<div class="style8" align="left">

Sp_92P5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

120kb

</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

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<div class="style8" align="left">

Eve

</div>
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<div class="style8" align="left">

Sp_79A2

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<div class="style8" align="left">

GFP,Cherry,H2B Cerulean

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<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131510 AC131510]

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158kb

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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Fbn3_2

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<div class="style8" align="left">

Sp_R33090B11

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

WHL22.145770

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<div class="style8" align="left">

SPU_014065

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</div>
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<div class="style8" align="left">

S.purpuratus

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<div class="style8" align="left">

Fbox

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<div class="style8" align="left">

Sp_038F09_L

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</div>
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<div class="style8" align="left">

</div>
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</div>
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</div>
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</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

FcgbpL

</div>
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<div class="style8" align="left">

Sp_3027D15

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<div class="style8" align="left">

GFP

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

145kb

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<div class="style8" align="left">

WHL22.23776

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<div class="style8" align="left">

SPU_000439

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

S.purpuratus

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<div class="style8" align="left">

Fmo2

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<div class="style8" align="left">

Sp_055A17_L

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146985 AC146985]

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</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Fmo3

</div>
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<div class="style8" align="left">

Sp_072G21_L

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146986 AC146986]

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</div>
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</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Foxa

</div>
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<div class="style8" align="left">

Sp_41 I19

</div>
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<div class="style8" align="left">

GFP,RFP, Dendra, Cerulean

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

150kb

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</div>
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</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Foxb

</div>
|
<div class="style8" align="left">

Sp_114N15_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131247 AC131247]

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135kb

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<div class="style8" align="left">

WHL.22.743430

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</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

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<div class="style8" align="left">

Foxb

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<div class="style8" align="left">

Sp_R33087A22

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131247 AC131247]

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<div class="style8" align="left">

135kb

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<div class="style8" align="left">

WHL22.743430

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<div class="style8" align="left">

SPU_004551

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Foxc

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<div class="style8" align="left">

Sp_136D11_L

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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

Echinobase length:136kb

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S.purpuratus

</div>
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<div class="style8" align="left">

Foxc

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<div class="style8" align="left">

Sp_3062K23

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

237000

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</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

FoxD

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<div class="style8" align="left">

Sp_3053L6

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<div class="style8" align="left">

Cherry

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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

SPU_014418.1

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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

FoxF

</div>
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<div class="style8" align="left">

Sp_34M18

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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</div>
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<div class="style8" align="left">

</div>
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</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Foxg

</div>
|
<div class="style8" align="left">

Sp_3055A17

</div>
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<div class="style8" align="left">

GFP

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

200kb

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</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Foxg

</div>
|
<div class="style8" align="left">

Sp_3101O07

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Fox n2/3

</div>
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<div class="style8" align="left">

Sp_3088J6

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Foxn2/3

</div>
|
<div class="style8" align="left">

Sp_307J6

</div>
|
<div class="style8" align="left">

GFP

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

143kb

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</div>
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<div class="style8" align="left">

</div>
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</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Foxn2/3

</div>
|
<div class="style8" align="left">

Sp_3088J6

</div>
|
<div class="style8" align="left">

GFP

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

143kb

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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Foxp

</div>
|
<div class="style8" align="left">

Sp_3052G21

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Foxq2

</div>
|
<div class="style8" align="left">

Sp_3044A21

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

72kb

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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Foxy(cy)

</div>
|
<div class="style8" align="left">

Sp_1032H24

</div>
|
<div class="style8" align="left">

GFP

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

118kb

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Fray

</div>
|
<div class="style8" align="left">

Sp_3096N17, Sp_1014L2

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

WHL22.249962,WHL22.249953

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</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

FreD

</div>
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<div class="style8" align="left">

Sp_3027C3

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

121kb

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<div class="style8" align="left">

WHL22.427938

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Frzd

</div>
|
<div class="style8" align="left">

Sp_153L07_S

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Gatac

</div>
|
<div class="style8" align="left">

Sp_081C18_L

</div>
|
<div class="style8" align="left">

GFP

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|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Gata e

</div>
|
<div class="style8" align="left">

Sp_40I9

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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</div>
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<div class="style8" align="left">

</div>
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</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Gatae

</div>
|
<div class="style8" align="left">

Sp_040I09_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146984 AC146984]

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100kb

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</div>
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<div class="style8" align="left">

</div>
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</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Gatae

</div>
|
<div class="style8" align="left">

Sp_091A10_L

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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</div>
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</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Gcm

</div>
|
<div class="style8" align="left">

Sp_187M9

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Gcn2

</div>
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<div class="style8" align="left">

Sp_1130J9

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.244656

</div>
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<div class="style8" align="left">

SPU_025674

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Gelsolinlike

</div>
|
<div class="style8" align="left">

Sp_118E15_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131379 AC131379]

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Gelsolin putative

</div>
|
<div class="style8" align="left">

Sp_R332B3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC149964.2 AC149964.2]

</div>
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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

SPU_000296

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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
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<div class="style8" align="left">

Gsc

</div>
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<div class="style8" align="left">

Sp_3051B6

</div>
|
<div class="style8" align="left">

GFP,H2B Cerulean

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

201kb

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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hairy 2/4(Hes)

</div>
|
<div class="style8" align="left">

Sp_103B20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hao1_4

</div>
|
<div class="style8" align="left">

Sp_12F6

</div>
|
<div class="style8" align="left">

(no GFP)

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.329268

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hbn

</div>
|
<div class="style8" align="left">

Sp_3067M10

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hedgehog

</div>
|
<div class="style8" align="left">

Sp_4011N13

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

178kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hes

</div>
|
<div class="style8" align="left">

Sp_R346N1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC177340.1 AC177340.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_012918

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hesblik

</div>
|
<div class="style8" align="left">

Sp_R33095M1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC180573.1 AC180573.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_026488

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hesc

</div>
|
<div class="style8" align="left">

Sp_3120N1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hesc

</div>
|
<div class="style8" align="left">

Sp_7G5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

217kb

</div>
|
<div class="style8" align="left">

WHL22.235339

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hex

</div>
|
<div class="style8" align="left">

Sp_6P7

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

145kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hex

</div>
|
<div class="style8" align="left">

Sp_R33050O2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC181029.1 AC181029.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_017990

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hexb

</div>
|
<div class="style8" align="left">

Sp_3050O24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.364740

</div>
|
<div class="style8" align="left">

SPU_017990

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hgcn5

</div>
|
<div class="style8" align="left">

Sp_089M06_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hhex

</div>
|
<div class="style8" align="left">

Sp_3032G13

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

22.626418

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hhex

</div>
|
<div class="style8" align="left">

Sp_3080E21

</div>
|
<div class="style8" align="left">

GFP,Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

22.626418

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hibch

</div>
|
<div class="style8" align="left">

Sp_3061L15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

22.119352

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hmx

</div>
|
<div class="style8" align="left">

Sp_15F1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

121kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hmx

</div>
|
<div class="style8" align="left">

Sp_4004C20

</div>
|
<div class="style8" align="left">

GFP, cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

144kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf1

</div>
|
<div class="style8" align="left">

Sp_25L21

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

168kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf1like

</div>
|
<div class="style8" align="left">

Sp_3003N7

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf1like

</div>
|
<div class="style8" align="left">

Sp_42F2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf4

</div>
|
<div class="style8" align="left">

Sp_3045J21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf4

</div>
|
<div class="style8" align="left">

Sp_3095 J22

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf6

</div>
|
<div class="style8" align="left">

Sp_13D8

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

253kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf6

</div>
|
<div class="style8" align="left">

Sp_079H14_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hnf6

</div>
|
<div class="style8" align="left">

Sp_088I24_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox1113a_b

</div>
|
<div class="style8" align="left">

Sp_135O12_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131446 AC131446]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox 11/13b

</div>
|
<div class="style8" align="left">

Sp_103k2

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

160kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox 11/13b

</div>
|
<div class="style8" align="left">

Sp_119l11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

145kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox 11/13b

</div>
|
<div class="style8" align="left">

Sp_122G5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

160kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox 11/13b

</div>
|
<div class="style8" align="left">

Sp_135012

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

105kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox 11/13b

</div>
|
<div class="style8" align="left">

Sp_142P8

</div>
|
<div class="style8" align="left">

GFP,H2B Cerulean

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

192kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox 11/13b

</div>
|
<div class="style8" align="left">

Sp_22P14

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

180kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox 11/13b

</div>
|
<div class="style8" align="left">

Sp_4005C17

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

142kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hox7

</div>
|
<div class="style8" align="left">

Sp_13H13

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

236kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hps4L

</div>
|
<div class="style8" align="left">

Sp_R31111E20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.562382.0

</div>
|
<div class="style8" align="left">

SPU_025757

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hypp_2177

</div>
|
<div class="style8" align="left">

Sp_R318J6

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.643455

</div>
|
<div class="style8" align="left">

SPU_027439

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hypp_2998, Hypp_2999

</div>
|
<div class="style8" align="left">

Sp_17B17

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=MK371206 MK371206]

</div>
|
<div class="style8" align="left">

145kb

</div>
|
<div class="style8" align="left">

WHL.22.239379

</div>
|
<div class="style8" align="left">

SPU_018406

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hypp_2998, Hypp_2999

</div>
|
<div class="style8" align="left">

Sp_R335J4

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

145kb

</div>
|
<div class="style8" align="left">

WHL22.239379

</div>
|
<div class="style8" align="left">

SPU_018407

</div>
|
<div class="style8" align="left">

Echinobase length: 203kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Hypp_375

</div>
|
<div class="style8" align="left">

Sp_4001C1 ,Sp_1013A10 ,Sp_1108B10

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.69123

</div>
|
<div class="style8" align="left">

SPU_024491

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ia1

</div>
|
<div class="style8" align="left">

Sp_1031P15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ia1

</div>
|
<div class="style8" align="left">

Sp_75G4

</div>
|
<div class="style8" align="left">

cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Id

</div>
|
<div class="style8" align="left">

Sp_R33120H1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=%20AC176846.1 AC176846.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_021469

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Id

</div>
|
<div class="style8" align="left">

Sp_R336E

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=%20AC180233.1 AC180233.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_000791

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ig/Egf/Vwc

</div>
|
<div class="style8" align="left">

Sp_3080E2,Sp_3037J19

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.139301

</div>
|
<div class="style8" align="left">

SPU_017202

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Inturned

</div>
|
<div class="style8" align="left">

Sp_14H15, Sp_1102N20

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.391869

</div>
|
<div class="style8" align="left">

SPU_008070

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ipgm

</div>
|
<div class="style8" align="left">

Sp_3071M24

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.633329,WHL22.633332

</div>
|
<div class="style8" align="left">

SPU_010377

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ire1,Ern2L

</div>
|
<div class="style8" align="left">

Sp_R33118A23

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.270316.1

</div>
|
<div class="style8" align="left">

SPU_017184

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Irxa

</div>
|
<div class="style8" align="left">

Sp_3094G12

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

206kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Kakapo

</div>
|
<div class="style8" align="left">

Sp_012G10_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

KirrelL

</div>
|
<div class="style8" align="left">

R3-28J10-14544

</div>
|
<div class="style8" align="left">

Cherry, GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

130kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Krox

</div>
|
<div class="style8" align="left">

Sp_163O19

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131502 AC131502]

</div>
|
<div class="style8" align="left">

7093kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lefty

</div>
|
<div class="style8" align="left">

Sp_3105O6

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lhx2.9

</div>
|
<div class="style8" align="left">

Sp_1027H9

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

124kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lim

</div>
|
<div class="style8" align="left">

Sp_108P04_L

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131507 AC131507]

</div>
|
<div class="style8" align="left">

138kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Limk

</div>
|
<div class="style8" align="left">

Sp_R3_3110L19

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

22.431075.2

</div>
|
<div class="style8" align="left">

SPU_022207

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lmo4

</div>
|
<div class="style8" align="left">

Sp_1008K19 (or K10?)

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lmo4

</div>
|
<div class="style8" align="left">

Sp_3106F20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lmo4

</div>
|
<div class="style8" align="left">

Sp_3119D15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lmpx

</div>
|
<div class="style8" align="left">

Sp_010A10_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lmr

</div>
|
<div class="style8" align="left">

Sp_4001F24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

22.327381.0

</div>
|
<div class="style8" align="left">

SPU_006026

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Lox

</div>
|
<div class="style8" align="left">

Sp_1004F15

</div>
|
<div class="style8" align="left">

cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

MitF

</div>
|
<div class="style8" align="left">

Sp_1103B18

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

152kb

</div>
|
<div class="style8" align="left">

WHL.22.677144

</div>
|
<div class="style8" align="left">

SPU_008175

</div>
|
<div class="style8" align="left">

note

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Mrpl12lik

</div>
|
<div class="style8" align="left">

Sp_R31102E

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=%20AC179348.1 AC179348.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_024214

</div>
|
<div class="style8" align="left">

Echinobase length: 99kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Mrpl2

</div>
|
<div class="style8" align="left">

Sp_R33083L1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC180730.1 AC180730.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_009141

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130

</div>
|
<div class="style8" align="left">

Sp_R32B17, Sp_R3113E14,Sp_R316A11, Sp_R33019H3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.438943

</div>
|
<div class="style8" align="left">

SPU_002088

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130

</div>
|
<div class="style8" align="left">

Sp_R33055B12

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

135kb

</div>
|
<div class="style8" align="left">

WHL22.438943

</div>
|
<div class="style8" align="left">

SPU_002088

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130_1

</div>
|
<div class="style8" align="left">

Sp_R32B17

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130_1

</div>
|
<div class="style8" align="left">

Sp_R33055B12, Sp_R3113E14,Sp_R316A11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130r1

</div>
|
<div class="style8" align="left">

Sp_R32B17, Sp_R33055B12, Sp_R3113E14,Sp_R316A11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.438976

</div>
|
<div class="style8" align="left">

SPU_013822

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130r3

</div>
|
<div class="style8" align="left">

Sp_R311C23, Sp_R32B17, Sp_R33055B12, Sp_R3113E14,Sp_R316A11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.438997

</div>
|
<div class="style8" align="left">

SPU_013823

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130related

</div>
|
<div class="style8" align="left">

Sp_R31021C1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC179718.1 AC179718.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_014496

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130related

</div>
|
<div class="style8" align="left">

Sp_R32B1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC176140.1 AC176140.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_013822

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130related

</div>
|
<div class="style8" align="left">

Sp_R33092L1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC180600.1 AC180600.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_015763

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msp130related

</div>
|
<div class="style8" align="left">

Sp_R363K2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC179470.1 AC179470.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_014492

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Msx

</div>
|
<div class="style8" align="left">

Sp_R33010N1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC168725.2 AC168725.2]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_020565

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Mt14/MmpL7

</div>
|
<div class="style8" align="left">

Sp_4001O12,Sp_3065G23,Sp_37M11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.312130

</div>
|
<div class="style8" align="left">

SPU_028750

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Mt14/MmpL7

</div>
|
<div class="style8" align="left">

Sp_R34001O12

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.312033

</div>
|
<div class="style8" align="left">

SPU_028748

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

NA

</div>
|
<div class="style8" align="left">

Sp_R33071I16

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.148948

</div>
|
<div class="style8" align="left">

SPU_023291

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nanos2

</div>
|
<div class="style8" align="left">

Sp_1111E20

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

41kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nk1

</div>
|
<div class="style8" align="left">

Sp_066D22_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nk1

</div>
|
<div class="style8" align="left">

Sp_142B02_L

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

128kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nk2.2

</div>
|
<div class="style8" align="left">

Sp_4019 C23

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

187kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nk7

</div>
|
<div class="style8" align="left">

Sp_1029A14

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

186kb

</div>
|
<div class="style8" align="left">

WHL22.567485.0

</div>
|
<div class="style8" align="left">

SPU_022573

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nk7

</div>
|
<div class="style8" align="left">

Sp_115H6,1029A14,40L3,13L2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nk7

</div>
|
<div class="style8" align="left">

Sp_R340L3

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=MK371204 MK371204]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.567485

</div>
|
<div class="style8" align="left">

SPU_022573

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nkx21

</div>
|
<div class="style8" align="left">

Sp_131N15_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nkx32

</div>
|
<div class="style8" align="left">

Sp_1037P9

</div>
|
<div class="style8" align="left">

cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nodal

</div>
|
<div class="style8" align="left">

Sp_7F21

</div>
|
<div class="style8" align="left">

GFP, RFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

180kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

None

</div>
|
<div class="style8" align="left">

Sp_R34007M3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.239320

</div>
|
<div class="style8" align="left">

SPU_018403

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Not

</div>
|
<div class="style8" align="left">

Sp_157D03_L

</div>
|
<div class="style8" align="left">

Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

162kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Notch

</div>
|
<div class="style8" align="left">

Sp_191I13

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

171kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Notch

</div>
|
<div class="style8" align="left">

Sp_R317A1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC175935.1 AC175935.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_027006

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Notch

</div>
|
<div class="style8" align="left">

Sp_191I13_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131449 AC131449]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Npff2L4

</div>
|
<div class="style8" align="left">

Sp_115K10

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL.22.523272.0

</div>
|
<div class="style8" align="left">

SPU_025314

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nr1

</div>
|
<div class="style8" align="left">

Sp_R311I1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC181201.1 AC181201.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_028255

</div>
|
<div class="style8" align="left">

Echinobase length: 75kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Nrl

</div>
|
<div class="style8" align="left">

Sp_007C10_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Orct

</div>
|
<div class="style8" align="left">

Sp_095C14_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131514 AC131514]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Osr

</div>
|
<div class="style8" align="left">

Sp_63M1

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

142kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Otx

</div>
|
<div class="style8" align="left">

Sp_006F13_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131452 AC131452]

</div>
|
<div class="style8" align="left">

161kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Otx

</div>
|
<div class="style8" align="left">

Sp_6F13ex6

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

162kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

P16

</div>
|
<div class="style8" align="left">

Sp_1113K17

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

120kb

</div>
|
<div class="style8" align="left">

WHL.22.239481

</div>
|
<div class="style8" align="left">

SPU_018408

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

P16

</div>
|
<div class="style8" align="left">

Sp_R31037B24

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.239481

</div>
|
<div class="style8" align="left">

SPU_018408

</div>
|
<div class="style8" align="left">

Echinobase length: 148kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

P58_a

</div>
|
<div class="style8" align="left">

Sp_3027D15

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

145kb

</div>
|
<div class="style8" align="left">

WHL.22.23776

</div>
|
<div class="style8" align="left">

SPU_00439

</div>
|
<div class="style8" align="left">

Echinobase length: 167kb.Same clone and WHL as FcgbpL

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

P58_a

</div>
|
<div class="style8" align="left">

Sp_R33077H02

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

145kb

</div>
|
<div class="style8" align="left">

WHL22.23776

</div>
|
<div class="style8" align="left">

SPU_004159

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pak(p21)ph

</div>
|
<div class="style8" align="left">

Sp_4004H24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=MK371209 MK371209]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL.22.294876

</div>
|
<div class="style8" align="left">

SPU_003534

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pax2/5/8

</div>
|
<div class="style8" align="left">

Sp_1038B19

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

Echinobase length: 83kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pax2/5/8

</div>
|
<div class="style8" align="left">

Sp_4029I17

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pbx

</div>
|
<div class="style8" align="left">

Sp_53H21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pdgfr/vegfrL

</div>
|
<div class="style8" align="left">

Sp_R33090K8

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.240520

</div>
|
<div class="style8" align="left">

SPU_000310

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pdgfr/vegfrlik

</div>
|
<div class="style8" align="left">

Sp_R33055P1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC178040.1 AC178040.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_021021

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pks

</div>
|
<div class="style8" align="left">

Sp_080H21_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131453 AC131453]

</div>
|
<div class="style8" align="left">

144kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pks

</div>
|
<div class="style8" align="left">

Sp_085B07_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

PKS

</div>
|
<div class="style8" align="left">

Sp_80H21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC178793.1 AC178793.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_028395

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

PKS

</div>
|
<div class="style8" align="left">

Sp_R321O1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC178793.1 AC178793.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_028395

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Plxdc2, Plxdc2_1

</div>
|
<div class="style8" align="left">

Sp_3061I23

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.605782

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pm27

</div>
|
<div class="style8" align="left">

Sp_074F08_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pm27

</div>
|
<div class="style8" align="left">

Sp_140A02_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pmar1

</div>
|
<div class="style8" align="left">

Sp_R31018E1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC179748.1 AC179748.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_015828

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pmar1

</div>
|
<div class="style8" align="left">

Sp_R315A

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC149920.2 AC149920.2]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_015829

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Pmar1

</div>
|
<div class="style8" align="left">

Sp_R34013A1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC168388.2 AC168388.2]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_014721

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Polyabinding

</div>
|
<div class="style8" align="left">

Sp_073E18_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Protocad15L

</div>
|
<div class="style8" align="left">

R31003A8

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

120kb

</div>
|
<div class="style8" align="left">

WHL22.138023

</div>
|
<div class="style8" align="left">

SPU_003730

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Prox

</div>
|
<div class="style8" align="left">

Sp_3106N18

</div>
|
<div class="style8" align="left">

GFP,H2B

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

186kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ptpriz

</div>
|
<div class="style8" align="left">

Sp_16L6

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

120kb

</div>
|
<div class="style8" align="left">

WHL.22.416979.0

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ptpriz

</div>
|
<div class="style8" align="left">

Sp_5E5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=MK371207 MK371207]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL.22.416979.0

</div>
|
<div class="style8" align="left">

SPU_023115

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

RigiL8

</div>
|
<div class="style8" align="left">

Sp_7A24, Sp_5B24

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Rx

</div>
|
<div class="style8" align="left">

Sp_3067M20 or (M10)

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Samd9L_2

</div>
|
<div class="style8" align="left">

Sp_R31107I17

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.254949

</div>
|
<div class="style8" align="left">

SPU_005515

</div>
|
<div class="style8" align="left">

Echinobase length: 159kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sc1

</div>
|
<div class="style8" align="left">

Sp_1018N24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

122kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sc1

</div>
|
<div class="style8" align="left">

Sp_175G9

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

Echinobase length: 159kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sc1

</div>
|
<div class="style8" align="left">

Sp_3097G9

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

267kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sc1

</div>
|
<div class="style8" align="left">

Sp_54D1

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

SerpL11

</div>
|
<div class="style8" align="left">

Sp_3044E1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.332375

</div>
|
<div class="style8" align="left">

SPU_004543

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Shr2

</div>
|
<div class="style8" align="left">

Sp_20B21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

111

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sip1

</div>
|
<div class="style8" align="left">

Sp_4026F15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Six1/2

</div>
|
<div class="style8" align="left">

Sp_1026L10

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

168kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Six1/2

</div>
|
<div class="style8" align="left">

Sp_3058B7

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

168kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Six 3

</div>
|
<div class="style8" align="left">

Sp_1040G11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Six 3

</div>
|
<div class="style8" align="left">

Sp_3045N20

</div>
|
<div class="style8" align="left">

Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

92kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Six 3

</div>
|
<div class="style8" align="left">

Sp_304 N20

</div>
|
<div class="style8" align="left">

cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

92kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Slc2a5

</div>
|
<div class="style8" align="left">

Sp_R35M11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

95kb

</div>
|
<div class="style8" align="left">

WHL22.692383

</div>
|
<div class="style8" align="left">

SPU_027868

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm29

</div>
|
<div class="style8" align="left">

Sp_R35D23

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

60kb

</div>
|
<div class="style8" align="left">

WHL22.39470

</div>
|
<div class="style8" align="left">

SPU_005990

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm30

</div>
|
<div class="style8" align="left">

Sp_R33049J

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC177965.1 AC177965.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_000826

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm30

</div>
|
<div class="style8" align="left">

Sp_R34009E1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC179070.1 AC179070.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_004869

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm30c

</div>
|
<div class="style8" align="left">

Sp_4009E11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=MK371205 MK371205]

</div>
|
<div class="style8" align="left">

34kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

Echinobase length: 80kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm30c

</div>
|
<div class="style8" align="left">

Sp_R31019K19

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.518121

</div>
|
<div class="style8" align="left">

SPU_000827

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm30E

</div>
|
<div class="style8" align="left">

Sp_R34009E11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

100kb

</div>
|
<div class="style8" align="left">

WHL22.70610

</div>
|
<div class="style8" align="left">

SPU_004867

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm37

</div>
|
<div class="style8" align="left">

Sp_3112c9

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.742403

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Sm50

</div>
|
<div class="style8" align="left">

Sp_74F8

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

144kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Soxb2

</div>
|
<div class="style8" align="left">

Sp_058L24_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131509 AC131509]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Soxc

</div>
|
<div class="style8" align="left">

Sp_3114N15

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

159kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Soxe

</div>
|
<div class="style8" align="left">

Sp_3014I4

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

110kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

SpClectin

</div>
|
<div class="style8" align="left">

Sp_R339A20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.728834

</div>
|
<div class="style8" align="left">

SPU_007882

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

SpClectin

</div>
|
<div class="style8" align="left">

Sp_R34012A17

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.728834

</div>
|
<div class="style8" align="left">

SPU_007882

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Spnsh1

</div>
|
<div class="style8" align="left">

Sp_1008F15

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.60674.1

</div>
|
<div class="style8" align="left">

SPU_000847

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

SpPpap2b

</div>
|
<div class="style8" align="left">

Sp_R363M7

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.310985

</div>
|
<div class="style8" align="left">

SPU_011127

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Srcr185

</div>
|
<div class="style8" align="left">

Sp_R34014J20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.253874

</div>
|
<div class="style8" align="left">

SPU_022285

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Synb

</div>
|
<div class="style8" align="left">

Sp_16D6

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

130kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Synb

</div>
|
<div class="style8" align="left">

Sp_56D3

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

150kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tbrain

</div>
|
<div class="style8" align="left">

Sp_031J08_L

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131381 AC131381]

</div>
|
<div class="style8" align="left">

137kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tbx 2/3

</div>
|
<div class="style8" align="left">

Sp_45A14

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tbx6/1

</div>
|
<div class="style8" align="left">

Sp_R34002B2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC168415.2 AC168415.2]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_020345

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tead4

</div>
|
<div class="style8" align="left">

Sp_1111C11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tel

</div>
|
<div class="style8" align="left">

Sp_3122C5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

TgfbrtII

</div>
|
<div class="style8" align="left">

Sp_3054B11, Sp_1035B22(noGFP)

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.191099.0

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

TgfbrtII

</div>
|
<div class="style8" align="left">

Sp_R33054B11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.191099.0

</div>
|
<div class="style8" align="left">

SPU_017511

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tgif

</div>
|
<div class="style8" align="left">

Sp_1028K14

</div>
|
<div class="style8" align="left">

Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

190kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tgif

</div>
|
<div class="style8" align="left">

Sp_3014J14 (or 3114J14?)

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

107kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tho1

</div>
|
<div class="style8" align="left">

Sp_R31037M09

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.614092

</div>
|
<div class="style8" align="left">

SPU_007382

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tim1

</div>
|
<div class="style8" align="left">

Sp_16N12

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tk8

</div>
|
<div class="style8" align="left">

Sp_R315I1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tk8

</div>
|
<div class="style8" align="left">

Sp_R315I2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.147868

</div>
|
<div class="style8" align="left">

SPU_004747

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tk8

</div>
|
<div class="style8" align="left">

Sp_R33076H20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tk8

</div>
|
<div class="style8" align="left">

Sp_R33076M20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tk8

</div>
|
<div class="style8" align="left">

Sp_R33085F05

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tll

</div>
|
<div class="style8" align="left">

Sp_3114F24

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

11kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tmem68

</div>
|
<div class="style8" align="left">

Sp_3120J18

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.629570

</div>
|
<div class="style8" align="left">

SPU_028851

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tob

</div>
|
<div class="style8" align="left">

Sp_3121D18

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL.22.145164

</div>
|
<div class="style8" align="left">

SPU_016792

</div>
|
<div class="style8" align="left">

Echinobase length: 110000kb

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Tollip_1

</div>
|
<div class="style8" align="left">

Sp_R33069K18

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.544727

</div>
|
<div class="style8" align="left">

SPU_026252

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Trim2_1

</div>
|
<div class="style8" align="left">

Sp_R34P15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.742590

</div>
|
<div class="style8" align="left">

SPU_020546

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Trpm3_3 (W06)

</div>
|
<div class="style8" align="left">

Sp_R344J22

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

130kb

</div>
|
<div class="style8" align="left">

WHL22.540206

</div>
|
<div class="style8" align="left">

SPU_018142

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Trpm3_4

</div>
|
<div class="style8" align="left">

Sp_R34H20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.652421

</div>
|
<div class="style8" align="left">

SPU_011587

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Ubiquitin

</div>
|
<div class="style8" align="left">

Sp_5B3

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

150kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Unc4.1

</div>
|
<div class="style8" align="left">

Sp_3005N3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Unc4.1

</div>
|
<div class="style8" align="left">

Sp_3032G21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Unc4.1

</div>
|
<div class="style8" align="left">

Sp_3060A11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Unc4.1

</div>
|
<div class="style8" align="left">

Sp_3060A21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

UnivinBMP2.4

</div>
|
<div class="style8" align="left">

Sp_1108 H16

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

UnivinBMP2.4

</div>
|
<div class="style8" align="left">

Sp_4013 P18

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Vasa

</div>
|
<div class="style8" align="left">

Sp_1032F3

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

159kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Vegf

</div>
|
<div class="style8" align="left">

Sp_R31109H

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC181571.1 AC181571.1]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

SPU_005737

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Vegf2

</div>
|
<div class="style8" align="left">

Sp_3040L10

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.194484

</div>
|
<div class="style8" align="left">

SPU_005737

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Vegf3 Erica

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Vegf3 Julie

</div>
|
<div class="style8" align="left">

Sp_4045B11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

78kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

VegFr

</div>
|
<div class="style8" align="left">

Sp_R33007G22

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.240520

</div>
|
<div class="style8" align="left">

SPU_000310

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Vparpf_3

</div>
|
<div class="style8" align="left">

Sp_4015K5

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Wnt5

</div>
|
<div class="style8" align="left">

Sp_1109L17

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

135kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Wnt8

</div>
|
<div class="style8" align="left">

Sp_040I09_L|Sp_041A08_S

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131383 AC131383]

</div>
|
<div class="style8" align="left">

78kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Wnt8

</div>
|
<div class="style8" align="left">

Sp_099F11_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131454 AC131454]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Z160

</div>
|
<div class="style8" align="left">

Sp_10K16

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.6653.0

</div>
|
<div class="style8" align="left">

SPU_018075

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Z160

</div>
|
<div class="style8" align="left">

Sp_3J11, Sp_10K16,Sp_1022C20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

WHL22.6653

</div>
|
<div class="style8" align="left">

SPU_018075

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Z166

</div>
|
<div class="style8" align="left">

Sp_3086D15

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

105kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Z167

</div>
|
<div class="style8" align="left">

Sp_15N2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Z188

</div>
|
<div class="style8" align="left">

Sp_3053B21

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

217kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.purpuratus

</div>
|
<div class="style8" align="left">

Z199 (Sp5)

</div>
|
<div class="style8" align="left">

Sp_4014H5

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Blimp1

</div>
|
<div class="style8" align="left">

Sf_1137N7

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Brachyury

</div>
|
<div class="style8" align="left">

Sf_124O17

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

100kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Brachyury

</div>
|
<div class="style8" align="left">

Sf_24O7

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

100kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Brn1/2/4

</div>
|
<div class="style8" align="left">

Sf_13A7, Sf_10K23, Sf_21K17, Sf_33H3, Sf_33H4, Sf_53H1, Sf_56C1, Sf_56N22, Sf_63B19, Sf_68M12, Sf_76C13, Sf_80I21, Sf_82P14

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Delta

</div>
|
<div class="style8" align="left">

Sf_135H4

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Dlx

</div>
|
<div class="style8" align="left">

Sf_126E15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Endo16

</div>
|
<div class="style8" align="left">

Sf_140P23

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Erg

</div>
|
<div class="style8" align="left">

Sf_156H6, Sf_61I12, Sf_71H11, Sf_108K22, Sf_114N21, Sf_267G13

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Eve

</div>
|
<div class="style8" align="left">

Sf_113P9

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

FoxA

</div>
|
<div class="style8" align="left">

Sf_121I13

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Gatae

</div>
|
<div class="style8" align="left">

Sf_121N5

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Gatae

</div>
|
<div class="style8" align="left">

Sf_21N5 Julies list

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Gcm

</div>
|
<div class="style8" align="left">

Sf_1190M4

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Hox11/13b

</div>
|
<div class="style8" align="left">

Sf_186O23, Sf_114F5, Sf_183B16

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

IrxA

</div>
|
<div class="style8" align="left">

Sf_126G6, Sf_56N10

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Nk2.2

</div>
|
<div class="style8" align="left">

Sf_156K11 70B18 76M21 116N10

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Otx

</div>
|
<div class="style8" align="left">

Sf_148I10

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Tbrain

</div>
|
<div class="style8" align="left">

Sf_1150J8

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

S.franciscanus

</div>
|
<div class="style8" align="left">

Wnt8

</div>
|
<div class="style8" align="left">

Sf_12H20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

P.lividus

</div>
|
<div class="style8" align="left">

Hbox12

</div>
|
<div class="style8" align="left">

Pl_020N20_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

P.lividus

</div>
|
<div class="style8" align="left">

Lim

</div>
|
<div class="style8" align="left">

Pl_070B09_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

P.lividus

</div>
|
<div class="style8" align="left">

Nodal

</div>
|
<div class="style8" align="left">

Pl_237K10 (127K10 Julie)

</div>
|
<div class="style8" align="left">

GFP, Cherry

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Alx1

</div>
|
<div class="style8" align="left">

Lv_7 J11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

162kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Apobec

</div>
|
<div class="style8" align="left">

Lv_031L12_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131495 AC131495]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

Echinobase length:51kb

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Apobec

</div>
|
<div class="style8" align="left">

Lv_265E21_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Apol

</div>
|
<div class="style8" align="left">

Lv_161M17_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Betacatenin

</div>
|
<div class="style8" align="left">

Lv_048J05_S, Lv_48J5

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131499 AC131499]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Blimp1

</div>
|
<div class="style8" align="left">

Lv_60B16

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Blimp1/krox

</div>
|
<div class="style8" align="left">

Lv_060B16_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131502 AC131502]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Bmp2/4

</div>
|
<div class="style8" align="left">

Lv_048J05_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131499 AC131499]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Brachyury

</div>
|
<div class="style8" align="left">

Lv_104N16_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146990 AC146990]

</div>
|
<div class="style8" align="left">

139kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Brachyury

</div>
|
<div class="style8" align="left">

Lv_187F03_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131486 AC131486]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Brn1 2/4

</div>
|
<div class="style8" align="left">

Lv_1I8

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Brn1 2/4

</div>
|
<div class="style8" align="left">

Lv_86D12

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Capk

</div>
|
<div class="style8" align="left">

Lv_086E23_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146991 AC146991]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Capk

</div>
|
<div class="style8" align="left">

Lv_177F13_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Cyclophillin

</div>
|
<div class="style8" align="left">

Lv_007J20_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Dac

</div>
|
<div class="style8" align="left">

Lv_259P14, Lv_263F11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Decorin

</div>
|
<div class="style8" align="left">

Lv_114C22_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131377 AC131377]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Delta

</div>
|
<div class="style8" align="left">

Lv_129M22_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146987 AC146987]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Delta

</div>
|
<div class="style8" align="left">

Lv_020B17_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Delta

</div>
|
<div class="style8" align="left">

Lv_174J14_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Dlx

</div>
|
<div class="style8" align="left">

Lv_17M23, Lv_93K13

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Dopt

</div>
|
<div class="style8" align="left">

Lv_037C14_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131497 AC131497]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Dri

</div>
|
<div class="style8" align="left">

Lv_6L12, Lv_147N11

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

Echinobase length:169kb.80130 smear

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Ecr

</div>
|
<div class="style8" align="left">

Lv_232F19

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Endo16

</div>
|
<div class="style8" align="left">

Lv_199M10_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC160519 AC160519]

</div>
|
<div class="style8" align="left">

22kb

</div>
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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Ese

</div>
|
<div class="style8" align="left">

Lv_260N2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Ets1

</div>
|
<div class="style8" align="left">

Lv_31F19

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

132kb

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Eve

</div>
|
<div class="style8" align="left">

Lv_112E15_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131376 AC131376]

</div>
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<div class="style8" align="left">

</div>
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</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Fmo2

</div>
|
<div class="style8" align="left">

Lv_223C01

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146993 AC146993]

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

Echinobase length:167kb.Same clone and WHL as P58a

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Fmo3

</div>
|
<div class="style8" align="left">

Lv_213G22

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Foxa

</div>
|
<div class="style8" align="left">

Lv_004G18_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131500 AC131500]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Foxa

</div>
|
<div class="style8" align="left">

Lv_46J3

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146988 AC146988]

</div>
|
<div class="style8" align="left">

133kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Foxb

</div>
|
<div class="style8" align="left">

Lv_092H22_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131504 AC131504]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Foxg

</div>
|
<div class="style8" align="left">

Lv_15K23

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Foxk

</div>
|
<div class="style8" align="left">

Lv_186I1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
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<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Foxn2/3

</div>
|
<div class="style8" align="left">

Lv_206M1; Lv_206C22: Lv_217D12

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Foxo

</div>
|
<div class="style8" align="left">

Lv_13C1; Lv_192P13

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Gatac

</div>
|
<div class="style8" align="left">

Lv_044D13_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Gatac

</div>
|
<div class="style8" align="left">

Lv_43P8

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Gatae

</div>
|
<div class="style8" align="left">

Lv_159L22_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC146989 AC146989]

</div>
|
<div class="style8" align="left">

135kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Gatae

</div>
|
<div class="style8" align="left">

Lv_032P20_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131496 AC131496]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Gcm

</div>
|
<div class="style8" align="left">

Lv_018J03_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131487 AC131487]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Gelsolin

</div>
|
<div class="style8" align="left">

Lv_066E20_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Gsc

</div>
|
<div class="style8" align="left">

Lv_157F2; Lv_188E3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Hes

</div>
|
<div class="style8" align="left">

Lv_091N14_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Hmx

</div>
|
<div class="style8" align="left">

Lv_076P20_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131503 AC131503]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Hnf1

</div>
|
<div class="style8" align="left">

Lv_252B16; 252C17

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Hnf6

</div>
|
<div class="style8" align="left">

Lv_015K10_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Hox1113a

</div>
|
<div class="style8" align="left">

Lv_235L15

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131563 AC131563]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Hox1113b

</div>
|
<div class="style8" align="left">

Lv_235L15_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131563 AC131563]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

IrxA

</div>
|
<div class="style8" align="left">

Lv_275N21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Kakapo

</div>
|
<div class="style8" align="left">

Lv_033E22_X

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Kakapo

</div>
|
<div class="style8" align="left">

Lv_229D15_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131492 AC131492]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Lim1

</div>
|
<div class="style8" align="left">

Lv_097A18_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131505 AC131505]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Nfe2

</div>
|
<div class="style8" align="left">

Lv_12M23; Lv_23F8; Lv_28N3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Nfkb

</div>
|
<div class="style8" align="left">

Lv_96M23

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Nk1

</div>
|
<div class="style8" align="left">

Lv_125K05_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Nk2.2

</div>
|
<div class="style8" align="left">

Lv_261O1; Lv_266P4; Lv_271G24

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Nodal

</div>
|
<div class="style8" align="left">

Lv_169O12

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Not

</div>
|
<div class="style8" align="left">

Lv_219N08_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Notch

</div>
|
<div class="style8" align="left">

Lv_030M02_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Notch

</div>
|
<div class="style8" align="left">

Lv_097A07_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Nrl

</div>
|
<div class="style8" align="left">

Lv_024M04_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131494 AC131494]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Otx

</div>
|
<div class="style8" align="left">

Lv_229L05_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131493 AC131493]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Pks

</div>
|
<div class="style8" align="left">

Lv_053J24_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131501 AC131501]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Pm27

</div>
|
<div class="style8" align="left">

Lv_188D08_L

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Pmar1

</div>
|
<div class="style8" align="left">

Lv_170H13_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131562 AC131562]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Profilin

</div>
|
<div class="style8" align="left">

Lv_048B05_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Prox

</div>
|
<div class="style8" align="left">

Lv_37H6; Lv_39I5; Lv_171G6

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Sip1

</div>
|
<div class="style8" align="left">

Lv_99D3; Lv_101P20; Lv_253P19

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Six1/2

</div>
|
<div class="style8" align="left">

Lv_58E21

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Snail (Z88)

</div>
|
<div class="style8" align="left">

Lv_9B9

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

SoxB1

</div>
|
<div class="style8" align="left">

Lv_153I1; 187G6

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Soxc

</div>
|
<div class="style8" align="left">

Lv_208L03_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131489 AC131489]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Tbr

</div>
|
<div class="style8" align="left">

Lv_192 I24

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Tbrain

</div>
|
<div class="style8" align="left">

Lv_192I24_S

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131488 AC131488]

</div>
|
<div class="style8" align="left">

551kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Tbx2/3

</div>
|
<div class="style8" align="left">

Lv_53J19; Lv_56A3

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Tel

</div>
|
<div class="style8" align="left">

Lv_270E1

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Wnt5

</div>
|
<div class="style8" align="left">

Lv_036N14_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Wnt8

</div>
|
<div class="style8" align="left">

Lv_064C04_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

127kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Wnt8

</div>
|
<div class="style8" align="left">

Lv_183H12_S

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=AC131485 AC131485]

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

L.variegatus

</div>
|
<div class="style8" align="left">

Z13

</div>
|
<div class="style8" align="left">

Lv_10B12

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

E.tribuloides

</div>
|
<div class="style8" align="left">

Eve

</div>
|
<div class="style8" align="left">

Et_161A22

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

120kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Brachyury

</div>
|
<div class="style8" align="left">

Ap_144G2

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

169kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Delta

</div>
|
<div class="style8" align="left">

Ap_158F20

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

36kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Eve

</div>
|
<div class="style8" align="left">

Ap_159E4

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

120kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Foxa

</div>
|
<div class="style8" align="left">

Ap_66L2

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Foxa

</div>
|
<div class="style8" align="left">

Ap_176M11_L

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

133kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Gatac

</div>
|
<div class="style8" align="left">

Ap_149G11

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

104kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Gatae

</div>
|
<div class="style8" align="left">

Ap_99O22

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

145kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Gcm

</div>
|
<div class="style8" align="left">

Ap_73C21

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

119kb

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Otx

</div>
|
<div class="style8" align="left">

Ap_22F20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Otx

</div>
|
<div class="style8" align="left">

Ap_46D17

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Tbrain

</div>
|
<div class="style8" align="left">

Ap_10E23

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

A.punctulata

</div>
|
<div class="style8" align="left">

Wnt8

</div>
|
<div class="style8" align="left">

Ap_38E20

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

</div>
|-
|
<div class="style8" align="left">

P.miniata

</div>
|
<div class="style8" align="left">

Brachyury

</div>
|
<div class="style8" align="left">

Am_187C19_S

</div>
|
<div class="style8" align="left">

GFP

</div>
|
<div class="style8" align="left">

</div>
|
<div class="style8" align="left">

90kb

</div>
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Delta

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Am_30B2

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Am_185N5

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Am_185F05_L

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Reporting bugs

2023-02-08T22:41:25Z

Xenbase:

=Reporting a bug in Echinobase=
If you find a bug in Echinobase, we would really like to know!
However, it is very important that we can find your bug so that we can fix it.

'''To help us find your bug, please fill out form below, and e-mail it to us at [mailto:echinobase@andrew.cmu.edu echinobase@andrew.cmu.edu].'''
<div class="toccolours mw-collapsible mw-collapsed">
Echinobase Bug Report
<div class="mw-collapsible-content">Date of bug- MM/DD/YYYY
approximate time at which the bug occurred: HH:MM:SS

Reporter:[your name]
URL at which the bug occurred:[Give the urls from the pages where you first felt that something was wrong]

1. What is the problem?
[A brief, one sentence explanation of the bug]

2. What were you trying to do?
[A brief explanation of what you were trying to do when the bug occurred]

3. Please give a step-by-step summary of the actions you took from the time you decided to perform the action in question 2 to the time you experienced the bug in question 1. If you were searching for something or changing the properties of something (i.e. changing a user's address), please include the exact text you entered (as far as you can remember).

4. On a scale of 1 to 6, how will this bug impact your ability to use the website? [Put your answer HERE]
[1= not at all]
[4= Working around this bug requires significant changes in how I use Echinobase]
[6= While this bug exists, I cannot use Echinobase to do what I need to do]</div>
</div>

Below you can find an example pre-filled bug report.
<div class="toccolours mw-collapsible mw-collapsed">
Echinobase Bug Report
<div class="mw-collapsible-content">
Date of bug- 04/11/2020
approximate time at which the bug occurred: 15:34:29

Reporter:John Smith
URL at which the bug occurred:http://www.echinobase.org/gene/searchGene.do?method=search&searchIn=0&searchValue=XPax-8&searchType=0

1. What is the problem?
I cannot search for genes with synonyms.

2. What were you trying to do?
I was trying to search for "pax2" with its synonym, "EPax-2"

3. Please give a step-by-step summary of the actions you took from the time you decided to perform the action in question 2 to the time you experienced the bug in question 1.If you were searching for something or changing the properties of something (i.e. changing a user's address), please include the exact text you entered (as far as you can remember).

1. I started at www.echinobase.org.
2. I entered "EPax-2" in the search box.
3. I received no results.

4. On a scale of 1 to 6, how will this bug impact your ability to use the website? 4

[1= not at all]
[4= Working around this bug requires significant changes in how I use Echinobase]
[6= While this bug exists, I cannot use Echinobase to do what I need to do]

</div>
</div>

An explanation of how to fill out each of the fields and why they are helpful, is below.

==Date and Time==
These fields are necessary when someone reports a bug that we cannot find the cause of. We can check Echinobase's error logs at that date and time to see if any errors occurred which might tell us more about the bug.

The seconds of the time field need only be approximations.

== 1. What is the Problem?==
Please provide a very short (1-2 sentence) summary of the problem.

== 2. What were you trying to do?==
This field gives us some context as to what you were attempting to do. It helps us determine which page you were on and pages you were likely to have been on before that.

== 3. Step-by-step Summary==
Write out, as well as you can remember, each and every step you took from the moment you decided to do the action in number 2 to the moment the bug from number 1 occurred.

===Why is this summary important?===
Some bugs may be crucial to Echinobase running correctly, but may only occur after a user has performed a very specific sequence of steps. The more specific you are about the steps you took (exactly which search box you used, where you reached an image edit page from, etc.)the easier it will be for us to isolate your bug.

An extreme example (unrelated to Echinobase) of how specific a bug report may need to be in order to isolate a bug can be found [http://computingcases.org/case_materials/therac/supporting_docs/therac_resources/produce_malfuction_54.html here]. Each and every step from that bug report would need to be performed correctly in order to generate that bug.

That being said, we understand your time is valuable and very few bugs actually require a report in the above level of detail. Just tell us which buttons you pushed and which pages you navigated to and if we need more information, we will e-mail you. :-)

==4. The Scale From 1 to 6==
Unfortunately we cannot get to every bug as soon as it is submitted. Some bugs need to wait in our bug queue before we can fix them. To help us prioritize the ordering of these bugs in the queue, we would like to know how inconvenient these bugs are for you. The scale is summarized in the bug report above; however, a more detailed scale summary can be found below.

#= The problem does not affect my ability to use the website.
#= I can easily work around the problem.
#= I can work around the problem.
#= I can work around the problem with some difficulty.
#= It is very difficult to work around the problem.
#= I cannot work around the problem.

----

Reporting bugs

2023-02-08T22:40:59Z

Xenbase: /* Reporting a bug in Echinobase */

=Reporting a bug in Echinobase=
If you find a bug in Echinobase, we would really like to know!
However, it is very important that we can find your bug so that we can fix it.

'''To help us find your bug, please fill out form below, and e-mail it to us at [mailto: echinobase@andrew.cmu.edu echinobase@andrew.cmu.edu].'''
<div class="toccolours mw-collapsible mw-collapsed">
Echinobase Bug Report
<div class="mw-collapsible-content">Date of bug- MM/DD/YYYY
approximate time at which the bug occurred: HH:MM:SS

Reporter:[your name]
URL at which the bug occurred:[Give the urls from the pages where you first felt that something was wrong]

1. What is the problem?
[A brief, one sentence explanation of the bug]

2. What were you trying to do?
[A brief explanation of what you were trying to do when the bug occurred]

3. Please give a step-by-step summary of the actions you took from the time you decided to perform the action in question 2 to the time you experienced the bug in question 1. If you were searching for something or changing the properties of something (i.e. changing a user's address), please include the exact text you entered (as far as you can remember).

4. On a scale of 1 to 6, how will this bug impact your ability to use the website? [Put your answer HERE]
[1= not at all]
[4= Working around this bug requires significant changes in how I use Echinobase]
[6= While this bug exists, I cannot use Echinobase to do what I need to do]</div>
</div>

Below you can find an example pre-filled bug report.
<div class="toccolours mw-collapsible mw-collapsed">
Echinobase Bug Report
<div class="mw-collapsible-content">
Date of bug- 04/11/2020
approximate time at which the bug occurred: 15:34:29

Reporter:John Smith
URL at which the bug occurred:http://www.echinobase.org/gene/searchGene.do?method=search&searchIn=0&searchValue=XPax-8&searchType=0

1. What is the problem?
I cannot search for genes with synonyms.

2. What were you trying to do?
I was trying to search for "pax2" with its synonym, "EPax-2"

3. Please give a step-by-step summary of the actions you took from the time you decided to perform the action in question 2 to the time you experienced the bug in question 1.If you were searching for something or changing the properties of something (i.e. changing a user's address), please include the exact text you entered (as far as you can remember).

1. I started at www.echinobase.org.
2. I entered "EPax-2" in the search box.
3. I received no results.

4. On a scale of 1 to 6, how will this bug impact your ability to use the website? 4

[1= not at all]
[4= Working around this bug requires significant changes in how I use Echinobase]
[6= While this bug exists, I cannot use Echinobase to do what I need to do]

</div>
</div>

An explanation of how to fill out each of the fields and why they are helpful, is below.

==Date and Time==
These fields are necessary when someone reports a bug that we cannot find the cause of. We can check Echinobase's error logs at that date and time to see if any errors occurred which might tell us more about the bug.

The seconds of the time field need only be approximations.

== 1. What is the Problem?==
Please provide a very short (1-2 sentence) summary of the problem.

== 2. What were you trying to do?==
This field gives us some context as to what you were attempting to do. It helps us determine which page you were on and pages you were likely to have been on before that.

== 3. Step-by-step Summary==
Write out, as well as you can remember, each and every step you took from the moment you decided to do the action in number 2 to the moment the bug from number 1 occurred.

===Why is this summary important?===
Some bugs may be crucial to Echinobase running correctly, but may only occur after a user has performed a very specific sequence of steps. The more specific you are about the steps you took (exactly which search box you used, where you reached an image edit page from, etc.)the easier it will be for us to isolate your bug.

An extreme example (unrelated to Echinobase) of how specific a bug report may need to be in order to isolate a bug can be found [http://computingcases.org/case_materials/therac/supporting_docs/therac_resources/produce_malfuction_54.html here]. Each and every step from that bug report would need to be performed correctly in order to generate that bug.

That being said, we understand your time is valuable and very few bugs actually require a report in the above level of detail. Just tell us which buttons you pushed and which pages you navigated to and if we need more information, we will e-mail you. :-)

==4. The Scale From 1 to 6==
Unfortunately we cannot get to every bug as soon as it is submitted. Some bugs need to wait in our bug queue before we can fix them. To help us prioritize the ordering of these bugs in the queue, we would like to know how inconvenient these bugs are for you. The scale is summarized in the bug report above; however, a more detailed scale summary can be found below.

#= The problem does not affect my ability to use the website.
#= I can easily work around the problem.
#= I can work around the problem.
#= I can work around the problem with some difficulty.
#= It is very difficult to work around the problem.
#= I cannot work around the problem.

----

Quantitative Expression Datasets and Plotting Tools

2022-06-23T17:27:48Z

Xenbase:

This page contains links to quantitative expression datasets and plotting tools, explore to find the tool that best suits your needs.
<p>For security reasons Echinobase <b>will not link</b> to these tools so do not click on the URL/http address.</p>
<p><b>Please COPY AND PASTE</b> the address (starting with http://) as text into the address bar of a new browser window to access the tool.</p>

<p>High Density Time Course Plotting Tool</p>
<p><nowiki>http://legacy.echinobase.org/hd-tc/plot.cgi</nowiki></p>

<p>Index to Expression Data and Integrated Plotting Tools</p>
<p><nowiki>http://legacy.echinobase.org/shiny/</nowiki></p>

<p>Nanostring Data and Plotting</p>
<p><nowiki>http://legacy.echinobase.org/shiny/nanostring/</nowiki></p>

Quantitative Expression Datasets and Plotting Tools

2022-06-23T17:27:11Z

Xenbase:

This page contains links to quantitative expression datasets and plotting tools, explore to find the tool that best suits your needs.
<p>For security reasons Echinobase <b>will not link</b> to these tools so do not click on the URL/http address.</p>
<p><b>Please COPY AND PASTE</b> the address (starting with http://) as text into the address bar of a new browser window to access the tool.</p>

<p>High Density Time Course Plotting Tool</p>
<p><nowiki>http://legacy.echinobase.org/hd-tc/plot.cgi</nowiki></p>

<p>Index to Expression Data and Integrated Plotting Tools</p>
<p>http://legacy.echinobase.org/shiny/</p>

<p>Nanostring Data and Plotting</p>
<p>http://legacy.echinobase.org/shiny/nanostring/</p>

Quantitative Expression Datasets and Plotting Tools

2022-06-23T17:25:53Z

Xenbase:

This page contains links to quantitative expression datasets and plotting tools, explore to find the tool that best suits your needs.
<p>For security reasons Echinobase <b>will not link</b> to these tools so do not click on the URL/http address.</p>
<p><b>Please COPY AND PASTE</b> the address (starting with http://) as text into the address bar of a new browser window to access the tool.</p>

<p>High Density Time Course Plotting Tool</p>
<p>http://legacy.echinobase.org/hd-tc/plot.cgi</p>

<p>Index to Expression Data and Integrated Plotting Tools</p>
<p>http://legacy.echinobase.org/shiny/</p>

<p>Nanostring Data and Plotting</p>
<p>http://legacy.echinobase.org/shiny/nanostring/</p>

Quantitative Expression Datasets and Plotting Tools

2022-06-23T17:25:44Z

Xenbase:

This page contains links to quantitative expression datasets and plotting tools, explore to find the tool that best suits your needs.
<p>For security reasons Echinobase <b>will not link</b> to these tools so do not click on the URL/http address.</p>
<p></p>
<p><b>Please COPY AND PASTE</b> the address (starting with http://) as text into the address bar of a new browser window to access the tool.</p>

<p>High Density Time Course Plotting Tool</p>
<p>http://legacy.echinobase.org/hd-tc/plot.cgi</p>

<p>Index to Expression Data and Integrated Plotting Tools</p>
<p>http://legacy.echinobase.org/shiny/</p>

<p>Nanostring Data and Plotting</p>
<p>http://legacy.echinobase.org/shiny/nanostring/</p>

Quantitative Expression Datasets and Plotting Tools

2022-06-23T17:25:05Z

Xenbase:

This page contains links to quantitative expression datasets and plotting tools, explore to find the tool that best suits your needs.
<p>For security reasons Echinobase <b>will not link</b> to these tools so do not click on the URL/http address.</p>
<p></p>
<p><b>Please COPY AND PASTE</b> the address (eg. http://) as text into the address bar of a new browser window to access the tool.</p>

<p>High Density Time Course Plotting Tool</p>
<p>http://legacy.echinobase.org/hd-tc/plot.cgi</p>

<p>Index to Expression Data and Integrated Plotting Tools</p>
<p>http://legacy.echinobase.org/shiny/</p>

<p>Nanostring Data and Plotting</p>
<p>http://legacy.echinobase.org/shiny/nanostring/</p>

File:Lv1.jpg

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